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  4. Recombinant Escherichia coli Sensor protein KdpD (kdpD), partial

Recombinant Escherichia coli Sensor protein KdpD (kdpD), partial

  • 中文名稱：
    大腸桿菌kdpD重組蛋白
  • 貨號：
    CSB-YP322039ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌kdpD重組蛋白
  • 貨號：
    CSB-EP322039ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌kdpD重組蛋白
  • 貨號：
    CSB-EP322039ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌kdpD重組蛋白
  • 貨號：
    CSB-BP322039ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌kdpD重組蛋白
  • 貨號：
    CSB-MP322039ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    kdpD
  • Uniprot No.：
  • 別名：
    kdpD; b0695; JW0683; Sensor protein KdpD; EC 2.7.13.3
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. KdpD may function as a membrane-associated protein kinase that phosphorylates KdpE in response to environmental signals.
  • 基因功能參考文獻：
    1. In this report authors provide evidence that the extracellular K(+) concentration serves as one of the stimuli sensed by KdpD. PMID: 23651428
    2. The ArcB and QseC transmembrane (TM) domains are both two-helical motifs, whereas the KdpD TM domain comprises a four-helical bundle with shorter second and third helices. The interhelical distances reveal weak interactions of all three receptors. PMID: 20498088
    3. Data show that kinase activity of KdpD is stimulated in its native membrane environment by fusion with liposomes of anionic, but reduced with liposomes of zwitterionic phospholipids. PMID: 19850005
    4. the sensing and response functions are both located in the C-terminal domain PMID: 16484207
    5. These data clearly show that the extension of the fourth transmembrane helix encompassing the arginine cluster is mainly involved in sensing both K+ limitation and osmotic upshift, which may not be separated mechanistically. PMID: 17704218
    6. Data indicate that stimulus perception by KdpD does not correlate with changes in the cytoplasmic volume, in the intracellular ATP or K(+) concentration. Data propose that a reduction in turgor cannot be the stimulus for KdpD. PMID: 18245296
    7. Dephosphorylated IIANtr enhances activity of the kdp promoter by direct binding of dephosphorylated IIA(Ntr) to the sensor kinase KdpD. PMID: 19400808
    8. The Usp domain within KdpD is important for proper KdpD/KdpE signaling. PMID: 19589130

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  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Universal stress protein A family
  • 數據庫鏈接：


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