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  4. Recombinant Escherichia coli Secretion monitor (secM)

Recombinant Escherichia coli Secretion monitor (secM)

  • 中文名稱：
    大腸桿菌secM重組蛋白
  • 貨號：
    CSB-YP351745ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌secM重組蛋白
  • 貨號：
    CSB-EP351745ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌secM重組蛋白
  • 貨號：
    CSB-EP351745ENV-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌secM重組蛋白
  • 貨號：
    CSB-BP351745ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌secM重組蛋白
  • 貨號：
    CSB-MP351745ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    secM
  • Uniprot No.：
  • 別名：
    secM; srrA; yacA; b0097; JW5007; Secretion monitor
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    38-170
  • 氨基酸序列
    AEP NAPAKATTRN HEPSAKVNFG QLALLEANTR RPNSNYSVDY WHQHAIRTVI RHLSFAMAPQ TLPVAEESLP LQAQHLALLD TLSALLTQEG TPSEKGYRID YAHFTPQAKF STPVWISQAQ GIRAGPQRLT
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Regulates secA expression by translational coupling of the secM secA operon. Ribosomes translating the C-terminal region of secM can disrupt an RNA repressor helix that normally blocks secA translation initiation, derepressing the expression of secA. Translational pausing of secM at Pro-166 under secretion-limiting conditions increases the duration of the disruption and thus increases secA expression. This is controlled by interaction of the secM signal peptide with secA and the translocon, possibly by secA pulling the paused secM out of the ribosome. The arrest sequence (150-FXXXXWIXXXXGIRAGP-166) is sufficient to cause arrest of unrelated proteins. Elongation arrest can be alleviated by mutations in the 23S rRNA or in ribosomal protein L22.
  • 基因功能參考文獻(xiàn)：
    1. SecM can regulate its own synthesis by the force generated during folding, tuning ribosome activity to structure acquisition by a nascent polypeptide PMID: 25908824
    2. Residues at positions between 161 and 166 of SecM showed decreased ribosome stalling. PMID: 21971705
    3. RNA SsrA tagging of secretory monitor SecM would rescue ribosomes to avoid excessive jamming of the translation apparatus on stop-less secM mRNA PMID: 15494397
    4. results suggest that cotranslational targeting of nascent SecM to the translocon plays previously unrecognized roles of facilitating the formation of functional SecA molecules PMID: 15713839
    5. compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled ribosome complex PMID: 16713583
    6. secM-encoded Pro166-tRNA functions as a nonpolypeptide element in fulfilling SecM's role as a secretion monitor. PMID: 16713584

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  • 亞細(xì)胞定位：
    Cytoplasm, cytosol. Periplasm. Note=The active form is cytosolic, while the periplasmic form is rapidly degraded, mainly by the tail-specific protease.
  • 蛋白家族：
    SecM family
  • 數(shù)據(jù)庫鏈接：


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