在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Escherichia coli Rhomboid protease glpG (glpG), partial

Recombinant Escherichia coli Rhomboid protease glpG (glpG), partial

  • 中文名稱：
    大腸桿菌glpG重組蛋白
  • 貨號：
    CSB-YP357664ENV1
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌glpG重組蛋白
  • 貨號：
    CSB-EP357664ENV1
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌glpG重組蛋白
  • 貨號：
    CSB-EP357664ENV1-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌glpG重組蛋白
  • 貨號：
    CSB-BP357664ENV1
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌glpG重組蛋白
  • 貨號：
    CSB-MP357664ENV1
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    glpG
  • Uniprot No.：
  • 別名：
    glpG; b3424; JW5687; Rhomboid protease GlpG; Intramembrane serine protease
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Rhomboid-type serine protease that catalyzes intramembrane proteolysis.
  • 基因功能參考文獻：
    1. we present a detailed protocol for optimization of expression and purification of three rhomboid proteases from Escherichia coli (ecGlpG), Haemophilus influenzae (hiGlpG), and Providencia stuartii (AarA). PMID: 28065266
    2. The rhomboid protease GlpG, was found to promote extraintestinal pathogenic Escherichia coli survival within the mouse intestinal tract in the presence of the intact natural microbiota. PMID: 28373355
    3. Unbinding of TM5 from the rest of the structure exposes GlpG's active site, consistent with studies of the catalytic mechanism of GlpG that suggest that TM5 serves as a substrate gate to the active site. PMID: 26858402
    4. Studied high-resolution structures of the catalytic transmembrane domain of the Escherichia coli GlpG rhomboid; this provided numerous insights that help explain how hydrolytic cleavage can be achieved below the membrane surface. PMID: 25307614
    5. performed kinetic folding and unfolding experiments on 69 mutants (engineered every 2-3 residues throughout the 178-residue transmembrane domain) of GlpG, a membrane-embedded rhomboid protease from Escherichia coli PMID: 26056273
    6. Peptidyl-chloromethylketones (CMKs) derived from the natural rhomboid substrate TatA from bacterium Providencia stuartii bind GlpG in a substrate-like manner, and their co-crystal structures with GlpG reveal the S1 to S4 subsites of the protease. PMID: 25216680
    7. Structure and mechanism of rhomboid protease. PMID: 23585569
    8. Data indicate that the rhomboid GlpG (ecGlpG) cytoplasmic domain exists as a dimer with extensive domain swapping between the two monomers. PMID: 23353827
    9. GlpG is a rhomboid membrane protease which recognizes features of transmembrane regions of substrates. Cleavage occurs between Ser & Asp in a region of high local hydrophilicity, which might be located juxtamembrane rather than intramembrane position PMID: 16216077
    10. 2.1 A resolution crystal structure of the GlpG core domain PMID: 17051161
    11. One of the two GlpG molecules in the asymmetric unit has an open conformation at the active site, with the transmembrane helix alpha5 bent away from the rest of the molecule PMID: 17099694
    12. the crystal structure of GlpG in a more open conformation, where the capping loop L5 has been lifted, exposing the previously buried and catalytically essential Ser-201 to outside aqueous solution PMID: 17277078
    13. The functional analyses have identified transmembrane helix 5 movement to gate lateral substrate entry as a rate-limiting step in intramembrane proteolysis. PMID: 17463085
    14. These results suggest that GlpG cleaves an extramembrane region of substrates, unlike the currently prevailing view that this class of membrane proteases acts against a membrane-embedded polypeptide segment. PMID: 17493126
    15. L1 loop is partially embedded in the membrane, and showed that alanine substitution of a highly preferred tryptophan (Trp136) at the distal tip of the L1 loop near the lipid:water interface reduced GlpG proteolytic activity. PMID: 17976648

    顯示更多

    收起更多

  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Peptidase S54 family
  • 數據庫鏈接：


主站蜘蛛池模板： 国产老熟女网站| 变态 另类 欧美 大码 日韩| 国产大屁股视频免费区| 国内精品美女视频免费直播| 亚洲欧美日韩中文字幕在线一区 | 人妻少妇无码专视频在线 | 日韩国产人妻一区二区三区| 免费无码黄网站在线看| 国产精品内射后入合集| 大战熟女丰满人妻av| 色8激情欧美成人久久综合电影| 玩弄丰满熟妇xxxxx性60| 午夜大片男女免费观看爽爽爽尤物 | 日韩人妻无码一区二区三区综合| 男女啪啪抽搐高潮动态图| 国产一区二区丝袜高跟鞋| 美女扒开大腿让男人桶| 国产肥熟女视频一区二区三区| 超碰曰口干天天种夜夜爽| 国产av福利久久| 福利姬国产精品一区在线| 免费吃奶摸下激烈视频| 国产精品久久久久久久久人妻 | 男女下面一进一出无遮挡| 国产精品自产拍高潮在线观看| 国产乱人伦偷精品视频免下载| 久久久欧美精品激情| 国产做a爰片久久毛片a片美国| 亚洲高清毛片一区二区| 欧美激情在线播放| 亚洲欧美日本久久综合网站点击| 曰本一道本久久88不卡| 久久国产亚洲精选av| 美女脱了内裤张开腿让男人桶网站| 妺妺窝人体色777777| 国产成人片无码免费视频软件| 亚洲一区二区三区av天堂| 中文字幕无码一区二区免费| 一本之道高清乱码久久久| 乌克兰性欧美精品高清| 中国女人内谢69xxxx免费视频|