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  4. Recombinant Escherichia coli Regulatory protein SdiA (sdiA)

Recombinant Escherichia coli Regulatory protein SdiA (sdiA)

  • 中文名稱：
    大腸桿菌sdiA重組蛋白
  • 貨號：
    CSB-YP357001ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌sdiA重組蛋白
  • 貨號：
    CSB-EP357001ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌sdiA重組蛋白
  • 貨號：
    CSB-EP357001ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌sdiA重組蛋白
  • 貨號：
    CSB-BP357001ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌sdiA重組蛋白
  • 貨號：
    CSB-MP357001ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    sdiA
  • Uniprot No.：
  • 別名：
    sdiA; b1916; JW1901; Regulatory protein SdiA
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-240
  • 氨基酸序列
    MQDKDFFSWR RTMLLRFQRM ETAEEVYHEI ELQAQQLEYD YYSLCVRHPV PFTRPKVAFY TNYPEAWVSY YQAKNFLAID PVLNPENFSQ GHLMWNDDLF SEAQPLWEAA RAHGLRRGVT QYLMLPNRAL GFLSFSRCSA REIPILSDEL QLKMQLLVRE SLMALMRLND EIVMTPEMNF SKREKEILRW TAEGKTSAEI AMILSISENT VNFHQKNMQK KINAPNKTQV ACYAAATGLI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Activates cell division by specifically increasing transcription from one of the two promoters that lie immediately upstream of the ftsQAZ gene cluster. Activates ydiV expression in response to extracellular autoinducer AI-1 (Vibrio fischeri autoinducer oxoC6).
  • 基因功能參考文獻：
    1. the characterization of a set of small, regulatory RNAs (sRNAs) expressed from an Escherichia coli locus we have denoted sdsN located adjacent to the LuxR-homolog gene sdiA, is reported. PMID: 27166377
    2. SdiA interacts with multiple species of effector ligands and its promoter recognition specificity is modulated to various directions depending on the interacting effectors. PMID: 24645791
    3. Crystal structures of intact SdiA are reported; the E. coli quorum-sensing receptor SdiA seems to bridge both intraspecies and interspecies communication. PMID: 24598739
    4. SdiA does not respond to indole but indole did inhibit N-acyl-l-homoserine lactone sensing by SdiA. PMID: 22610437
    5. CsrA represses translation of sdiA by binding to the coding region of sdiA mRNA. PMID: 21908661
    6. roles of SdiA in E. coli (and Salmonella typhimurium): cell division; antibiotic resistance; virulence; biofilms; acid resistance; gene expression regulation; microbial viability [sdiA; quorum-sensing transcriptional activator; [REVIEW] PMID: 21034261
    7. NMR analysis of assignments for Escherichia coli SdiA1-171 PMID: 15929010
    8. The three-dimensional structure of a complex between the N-terminal domain of SdiA and a candidate autoinducer N-octanoyl-L-homoserine lactone (C8-HSL) has been calculated in solution from NMR data. PMID: 16307757
    9. Wild-type SdiA decreased biofilm formation by reducing curli production and motility. PMID: 19168658

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