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  4. Recombinant Escherichia coli Redox-sensitive transcriptional activator soxR (soxR)

Recombinant Escherichia coli Redox-sensitive transcriptional activator soxR (soxR)

  • 中文名稱：
    大腸桿菌soxR重組蛋白
  • 貨號：
    CSB-YP364851ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌soxR重組蛋白
  • 貨號：
    CSB-EP364851ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌soxR重組蛋白
  • 貨號：
    CSB-EP364851ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌soxR重組蛋白
  • 貨號：
    CSB-BP364851ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌soxR重組蛋白
  • 貨號：
    CSB-MP364851ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    soxR
  • Uniprot No.：
  • 別名：
    soxR; marC; b4063; JW4024; Redox-sensitive transcriptional activator SoxR
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-154
  • 氨基酸序列
    MEKKLPRIKA LLTPGEVAKR SGVAVSALHF YESKGLITSI RNSGNQRRYK RDVLRYVAII KIAQRIGIPL ATIGEAFGVL PEGHTLSAKE WKQLSSQWRE ELDRRIHTLV ALRDELDGCI GCGCLSRSDC PLRNPGDRLG EEGTGARLLE DEQN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Activates the transcription of the soxS gene which itself controls the superoxide response regulon. SoxR contains a 2Fe-2S iron-sulfur cluster that may act as a redox sensor system that recognizes superoxide. The variable redox state of the Fe-S cluster is employed in vivo to modulate the transcriptional activity of SoxR in response to specific types of oxidative stress. Upon reduction of 2Fe-2S cluster, SoxR reversibly loses its transcriptional activity, but retains its DNA binding affinity.
  • 基因功能參考文獻：
    1. Data indicate reaction of nitric oxide with transcription factor SoxR by pulse radiolysis. PMID: 24747116
    2. This review focuses on the mechanisms by which SoxR proteins respond to oxidative stress. PMID: 24332474
    3. study compared responsiveness of SoxRs from Streptomyces coelicolor, Pseudomonas aeruginosa and E. coli towards redox-active compounds; ScSoxR is the most limited in the range of chemicals to which it responds and has highest reduction potential; both kinetic and redox potential factors determine range of effective chemicals PMID: 24112649
    4. Analysis of apo-SoxR binding determines that the effect of apo-SoxR on DNA triple-crossover molecules (TX) in the cohesive tract of a DNA-based mechanical device is similar to the effect of shortening the device by 2 base pairs. PMID: 22257222
    5. The results indicate that activation of the soxRS regulon in E. coli cells exposed to superoxide-propagating compounds can be triggered by depletion of the NADPH stock rather than accumulation of superoxide itself. PMID: 21178165
    6. Conformational changes induced upon reduction of DNA-bound SoxR are not significantly different from those of DNA-free SoxR. PMID: 21988663
    7. mutant SoxR proteins contributed to the multiple-antibiotic resistance phenotypes of the clinical strains and were sufficient to confer multiple-antibiotic resistance in a fresh genetic background PMID: 15980345

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