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Recombinant Escherichia coli RNA pyrophosphohydrolase (rppH)

  • 中文名稱:
    大腸桿菌rppH重組蛋白
  • 貨號:
    CSB-YP363964ENV
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大腸桿菌rppH重組蛋白
  • 貨號:
    CSB-EP363964ENV
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大腸桿菌rppH重組蛋白
  • 貨號:
    CSB-EP363964ENV-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大腸桿菌rppH重組蛋白
  • 貨號:
    CSB-BP363964ENV
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大腸桿菌rppH重組蛋白
  • 貨號:
    CSB-MP363964ENV
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    rppH
  • Uniprot No.:
  • 別名:
    rppH; nudH; ygdP; b2830; JW2798; RNA pyrophosphohydrolase; EC 3.6.1.-,; Di)nucleoside polyphosphate hydrolase; Ap5A pyrophosphatase
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-176
  • 氨基酸序列
    MIDDDGYRPN VGIVICNRQG QVMWARRFGQ HSWQFPQGGI NPGESAEQAM YRELFEEVGL SRKDVRILAS TRNWLRYKLP KRLVRWDTKP VCIGQKQKWF LLQLVSGDAE INMQTSSTPE FDGWRWVSYW YPVRQVVSFK RDVYRRVMKE FASVVMSLQE NTPKPQNASA YRRKRG
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Master regulator of 5'-end-dependent mRNA decay. Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage. Preferentially hydrolyzes diadenosine penta-phosphate with ATP as one of the reaction products. Also able to hydrolyze diadenosine hexa- and tetra-phosphate. Has no activity on diadenosine tri-phosphate, ADP-ribose, NADH and UDP-glucose. In an RNase PH (rph) wild-type strain background, RppH is not required for maturation of tRNAs; however, strains with the truncated rph allele (for example K12 W1485 and its derivatives MG1655 and W3110) require RppH for maturation of certain monocistronic tRNAs with short (<5 nucleotides) leader sequences. In the meningitis causing strain E.coli K1, has been shown to play a role in HBMEC (human brain microvascular endothelial cells) invasion in vitro.
  • 基因功能參考文獻:
    1. it was also determined that diphosphorylated mRNA decay intermediates are abundant in wild-type E. coli and that their fractional level increases to almost 100% for representative mRNAs in mutant cells lacking RppH. PMID: 29619898
    2. study examined phenotypes of the rppH mutant strain to define the physiological role of RppH in more detail; through various phenotypic analyses of the rppH mutant, found the rppH mutant cells have increased envelope permeability; propose that RppH is associated with regulation of envelope integrity PMID: 28859318
    3. Triphosphate-to-monophosphate conversion to induce 5' end-dependent RNA degradation is a two-step process in E. coli involving gamma-phosphate removal by an unidentified enzyme to enable subsequent beta-phosphate removal by RppH. PMID: 28673541
    4. Data show that the specificity of RNA pyrophosphohydrolase RppH is species-dependent. PMID: 25657006
    5. Data indicate that X-ray structures of RNA pyrophosphohydrolase RppH have revealed molecular features important for RNA recognition. PMID: 25657011
    6. The data suggest that the cellular level of DapF is a critical factor regulating the RppH-catalyzed pyrophosphate removal and the subsequent degradation of target mRNAs. PMID: 25313159
    7. RppH not only catalyses a process functionally reminiscent of eukaryotic mRNA decapping but also bears an evolutionary relationship to the eukaryotic decapping enzyme Dcp2 PMID: 18202662

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  • 蛋白家族:
    Nudix hydrolase family, RppH subfamily
  • 數據庫鏈接:


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