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  4. Recombinant Escherichia coli Putative ribosome biogenesis GTPase RsgA (rsgA)

Recombinant Escherichia coli Putative ribosome biogenesis GTPase RsgA (rsgA)

  • 中文名稱：
    大腸桿菌rsgA重組蛋白
  • 貨號：
    CSB-YP334541ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌rsgA重組蛋白
  • 貨號：
    CSB-EP334541ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌rsgA重組蛋白
  • 貨號：
    CSB-EP334541ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌rsgA重組蛋白
  • 貨號：
    CSB-BP334541ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌rsgA重組蛋白
  • 貨號：
    CSB-MP334541ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rsgA
  • Uniprot No.：
  • 別名：
    rsgA; engC; yjeQ; b4161; JW4122; Small ribosomal subunit biogenesis GTPase RsgA; EC 3.6.1.-
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-350
  • 氨基酸序列
    MSKNKLSKGQ QRRVNANHQR RLKTSKEKPD YDDNLFGEPD EGIVISRFGM HADVESADGD VHRCNIRRTI RSLVTGDRVV WRPGKPAAEG VNVKGIVEAV HERTSVLTRP DFYDGVKPIA ANIDQIVIVS AILPELSLNI IDRYLVACET LQIEPIIVLN KIDLLDDEGM AFVNEQMDIY RNIGYRVLMV SSHTQDGLKP LEEALTGRIS IFAGQSGVGK SSLLNALLGL QKEILTNDIS DNSGLGQHTT TAARLYHFPH GGDVIDSPGV REFGLWHLEP EQITQGFVEF HDYLGLCKYR DCKHDTDPGC AIREAVEEGK IAETRFENYH RILESMAQVK TRKNFSDTDD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Binds the 30S subunit contacting the head, platform, and rRNA helix 44, which may assist the last maturation stages. Removes RbfA from mature, but not immature 30S ribosomes in a GTP-dependent manner; 95% removal in the presence of GTP, 90% removal in GMP-PNP and 65% removal in the presence of GDP. Circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover. Dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96-fold increase in kcat) ribosomes. Mature 30S ribosomes stimulate intrinsic GTPase more than do immature 30S ribosomes. Ribosome-associated GTPase activity is stimulated by RbfA. The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin streptomycin and spectinomycin. This inhibition is not due to competition for binding sites on the 30S or 70S ribosome.
  • 基因功能參考文獻：
    1. This finding indicates that, along with acting as an assembly factor, YjeQ has a role as a checkpoint protein, consisting of testing the proofreading ability of the 30S subunit. The structure also informs the mechanism by which YjeQ implements the release from the 30S subunit of a second assembly factor, called RbfA. PMID: 28396444
    2. costructure revealed that YjeQ interacts simultaneously with helix 44, the head and the platform of the 30S subunit PMID: 21960487
    3. RsgA may be close contact with the A site of the decoding center of the small subunit of the ribosome. PMID: 18029743
    4. The work is most consistent with the hypothesis that YjeQ has a role in late 30S subunit biogenesis. PMID: 18223068
    5. Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells. PMID: 19620234

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRAFAC class YlqF/YawG GTPase family, RsgA subfamily
  • 數據庫鏈接：


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