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  2. 蛋白
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  4. Recombinant Escherichia coli Protein umuC (umuC)

Recombinant Escherichia coli Protein umuC (umuC)

  • 中文名稱：
    大腸桿菌umuC重組蛋白
  • 貨號(hào)：
    CSB-YP366108ENV
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌umuC重組蛋白
  • 貨號(hào)：
    CSB-EP366108ENV
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌umuC重組蛋白
  • 貨號(hào)：
    CSB-EP366108ENV-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌umuC重組蛋白
  • 貨號(hào)：
    CSB-BP366108ENV
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌umuC重組蛋白
  • 貨號(hào)：
    CSB-MP366108ENV
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    umuC
  • Uniprot No.：
  • 別名：
    umuC; b1184; JW1173; Protein UmuC; DNA polymerase V; Pol V
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-422
  • 氨基酸序列
    MFALCDVNAF YASCETVFRP DLWGKPVVVL SNNDGCVIAR NAEAKALGVK MGDPWFKQKD LFRRCGVVCF SSNYELYADM SNRVMSTLEE LSPRVEIYSI DEAFCDLTGV RNCRDLTDFG REIRATVLQR THLTVGVGIA QTKTLAKLAN HAAKKWQRQT GGVVDLSNLE RQRKLMSALP VDDVWGIGRR ISKKLDAMGI KTVLDLADTD IRFIRKHFNV VLERTVRELR GEPCLQLEEF APTKQEIICS RSFGERITDY PSMRQAICSY AARAAEKLRS EHQYCRFIST FIKTSPFALN EPYYGNSASV KLLTPTQDSR DIINAATRSL DAIWQAGHRY QKAGVMLGDF FSQGVAQLNL FDDNAPRPGS EQLMTVMDTL NAKEGRGTLY FAGQGIQQQW QMKRAMLSPR YTTRSSDLLR VK
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in UV protection and mutation. Poorly processive, error-prone DNA polymerase involved in translesion repair. Essential for induced (or SOS) mutagenesis. Able to replicate DNA across DNA lesions (thymine photodimers and abasic sites, translesion synthesis) in the presence of activated RecA; efficiency is maximal in the presence of the beta sliding-clamp and clamp-loading complex of DNA polymerase III plus single-stranded binding protein (SSB). RecA and to a lesser extent the beta clamp-complex may target Pol V to replication complexes stalled at DNA template lesions.
  • 基因功能參考文獻(xiàn)：
    1. This work reveals a new level of regulation in the activation of DNA polymerase V. pol V is subject to a novel form of spatial regulation. PMID: 26317348
    2. The UmuC peptide follows the overall disposition of previously characterised structures with respect to the highly conserved glutamine residue. PMID: 23822808
    3. Y11A UmuC mutant has increased UV-sensitivity and reduced UV-mutability due to excessive incorporation of rNTPs during translesion DNA synthesis. PMID: 22784977
    4. Variants of UmuC with amino acid substitutions at, or adjacent to, its steric gate were studied to gain insights into sugar selectivity and overall fidelity of E. coli pol V. PMID: 22422840
    5. In the absence of repair or when the repair capacity of the cell has been exceeded, translesion synthesis by polymerase V (Pol V) allows DNA synthesis to resume and is required to protect the arrested replication fork from degradation PMID: 16199565
    6. Lyase activity intrinsic to polymerase IV PMID: 16202661

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  • 蛋白家族：
    DNA polymerase type-Y family
  • 數(shù)據(jù)庫(kù)鏈接：


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