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Recombinant Escherichia coli Protein HypA (hypA)

  • 中文名稱:
    大腸桿菌hypA重組蛋白
  • 貨號:
    CSB-YP363930ENV
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大腸桿菌hypA重組蛋白
  • 貨號:
    CSB-EP363930ENV
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大腸桿菌hypA重組蛋白
  • 貨號:
    CSB-EP363930ENV-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大腸桿菌hypA重組蛋白
  • 貨號:
    CSB-BP363930ENV
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大腸桿菌hypA重組蛋白
  • 貨號:
    CSB-MP363930ENV
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    hypA
  • Uniprot No.:
  • 別名:
    hypA; b2726; JW2696; Hydrogenase maturation factor HypA
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-116
  • 氨基酸序列
    MHEITLCQRA LELIEQQAAK HGAKRVTGVW LKIGAFSCVE TSSLAFCFDL VCRGSVAEGC KLHLEEQEAE CWCETCQQYV TLLTQRVRRC PQCHGDMLQI VADDGLQIRR IEIDQE
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase. Mediates transfer of nickel, but not zinc, from the low-affinity metal-binding site in the GTPase domain of HypB to HypA. HypA is involved in maturation of hydrogenase 3. It may partially substitute for the function of HybF and vice versa. May act as a scaffold for assembly of the nickel insertion proteins with the hydrogenase precursor protein after delivery of the iron center.
  • 基因功能參考文獻:
    1. At different stages of the hydrogenase maturation process, HypA was observed at or near the cell membrane, as was HycE, suggesting membrane localization of the nickel insertion event. PMID: 22016389
  • 亞細胞定位:
    Cell inner membrane.
  • 蛋白家族:
    HypA/HybF family
  • 數據庫鏈接:


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