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  4. Recombinant Escherichia coli Periplasmic chaperone PpiD (ppiD), partial

Recombinant Escherichia coli Periplasmic chaperone PpiD (ppiD), partial

  • 中文名稱：
    大腸桿菌ppiD重組蛋白
  • 貨號：
    CSB-YP018474ENV1
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌ppiD重組蛋白
  • 貨號：
    CSB-EP018474ENV1
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌ppiD重組蛋白
  • 貨號：
    CSB-EP018474ENV1-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌ppiD重組蛋白
  • 貨號：
    CSB-BP018474ENV1
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌ppiD重組蛋白
  • 貨號：
    CSB-MP018474ENV1
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ppiD; ybaU; b0441; JW0431; Periplasmic chaperone PpiD; Periplasmic folding chaperone
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Chaperone that functions as a gatekeeper on the periplasmic side of the SecYEG translocon. Facilitates the translocation of precursor proteins across SecYEG by interacting with the translocating substrate. Also plays a role in the release of newly synthesized secreted proteins at the periplasmic exit site of the Sec translocon. May be involved in the early periplasmic folding of many newly translocated proteins. Acts as a transient subunit of the Sec translocon that binds to the lateral gate of SecY and is detached by nascent membrane proteins but not by SecA.; Does not have peptidyl-prolyl isomerase (PPIase) activity. PPIase activity could not be generated by substitutions at the peptide binding site of the isolated parvulin-like domain.; (Microbial infection) May play an important role in bacteriophage T4 infection and be involved in the penetration of the inner membrane by the bacteriophage injection machinery, resulting in a DNA-conducting channel to translocate the phage DNA into the interior of the cell.
  • 基因功能參考文獻：
    1. PpiD contributes to the efficient detachment of newly secreted OmpA from the inner membrane and in doing so, seems to cooperate in a hierarchical manner with other periplasmic chaperones such as SurA, DegP, and Skp. PMID: 29097228
    2. PpiD functions as a chaperone and contributes to the network of periplasmic chaperone activities PMID: 20920237
    3. although PpiD and SurA have partially overlapping substrate specificities, they fulfil different functions in the cell PMID: 18498364
  • 亞細胞定位：
    Cell inner membrane; Single-pass type II membrane protein; Periplasmic side.
  • 數(shù)據(jù)庫鏈接：


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