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  4. Recombinant Escherichia coli Outer-membrane lipoprotein carrier protein (lolA)

Recombinant Escherichia coli Outer-membrane lipoprotein carrier protein (lolA)

  • 中文名稱：
    大腸桿菌lolA重組蛋白
  • 貨號：
    CSB-YP350327ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌lolA重組蛋白
  • 貨號：
    CSB-EP350327ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌lolA重組蛋白
  • 貨號：
    CSB-EP350327ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌lolA重組蛋白
  • 貨號：
    CSB-BP350327ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌lolA重組蛋白
  • 貨號：
    CSB-MP350327ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    lolA
  • Uniprot No.：
  • 別名：
    lolA; lplA; yzzV; b0891; JW0874; Outer-membrane lipoprotein carrier protein; P20
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    22-203
  • 氨基酸序列
    DAASDLKSR LDKVSSFHAS FTQKVTDGSG AAVQEGQGDL WVKRPNLFNW HMTQPDESIL VSDGKTLWFY NPFVEQATAT WLKDATGNTP FMLIARNQSS DWQQYNIKQN GDDFVLTPKA SNGNLKQFTI NVGRDGTIHQ FSAVEQDDQR SSYQLKSQQN GAVDAAKFTF TPPQGVTVDD QRK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane); the inner membrane retention signal functions at the release step.; May act as a regulator of the RCS-phosphorelay signal transduction pathway.
  • 基因功能參考文獻：
    1. analysis of roles of the hydrophobic cavity and lid of LolA in the lipoprotein transfer reaction in Escherichia coli PMID: 16354671
    2. The membrane localization of lipoproteins with the LolA derivative lacking the 3(10) helix was independent of LolB whereas LolB was essential for the outer membrane localization of lipoproteins with the wild-type LolA. PMID: 18503771
    3. the modified weighed Chemical-Shift Index (CSI) method suggested that the secondary structure of LolA in solution generally corresponded to that in the crystal structure PMID: 19636845
  • 亞細胞定位：
    Periplasm.
  • 蛋白家族：
    LolA family
  • 數據庫鏈接：


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