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Recombinant Escherichia coli Nitrogen regulation protein NR (I)

  • 中文名稱:
    大腸桿菌glnG重組蛋白
  • 貨號:
    CSB-YP365286ENV
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大腸桿菌glnG重組蛋白
  • 貨號:
    CSB-EP365286ENV
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大腸桿菌glnG重組蛋白
  • 貨號:
    CSB-EP365286ENV-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大腸桿菌glnG重組蛋白
  • 貨號:
    CSB-BP365286ENV
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大腸桿菌glnG重組蛋白
  • 貨號:
    CSB-MP365286ENV
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    glnG
  • Uniprot No.:
  • 別名:
    glnG; glnT; ntrC; b3868; JW3839; DNA-binding transcriptional regulator NtrC; Nitrogen regulation protein NR(I); Nitrogen regulator I; NRI
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-469
  • 氨基酸序列
    MQRGIVWVVD DDSSIRWVLE RALAGAGLTC TTFENGAEVL EALASKTPDV LLSDIRMPGM DGLALLKQIK QRHPMLPVII MTAHSDLDAA VSAYQQGAFD YLPKPFDIDE AVALVERAIS HYQEQQQPRN VQLNGPTTDI IGEAPAMQDV FRIIGRLSRS SISVLINGES GTGKELVAHA LHRHSPRAKA PFIALNMAAI PKDLIESELF GHEKGAFTGA NTIRQGRFEQ ADGGTLFLDE IGDMPLDVQT RLLRVLADGQ FYRVGGYAPV KVDVRIIAAT HQNLEQRVQE GKFREDLFHR LNVIRVHLPP LRERREDIPR LARHFLQVAA RELGVEAKLL HPETEAALTR LAWPGNVRQL ENTCRWLTVM AAGQEVLIQD LPGELFESTV AESTSQMQPD SWATLLAQWA DRALRSGHQN LLSEAQPELE RTLLTTALRH TQGHKQEAAR LLGWGRNTLT RKLKELGME
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Phosphorylated NtrC binds directly to DNA and stimulates the formation of open promoter-sigma54-RNA polymerase complexes. Activates transcription of many genes and operons whose products minimize the slowing of growth under nitrogen-limiting conditions, including genes coding for glutamine synthetase (glnA), transporters, amino acid permeases and catabolic enzymes.
  • 基因功能參考文獻:
    1. Authors show that the dominant role of NtrC is to regulate glnA transcription and its own expression, indicating that the glutamine paradox is not due to NtrC-independent gene regulation. PMID: 24255125
    2. Results determine a possible pathway of interconversion between the active state and the inactive state of nitrogen regulatory protein C by targeted molecular dynamics simulations. PMID: 19576227
  • 亞細胞定位:
    Cytoplasm.
  • 數據庫鏈接:


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