在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Escherichia coli Negative modulator of initiation of replication (seqA)

Recombinant Escherichia coli Negative modulator of initiation of replication (seqA)

  • 中文名稱：
    大腸桿菌seqA重組蛋白
  • 貨號：
    CSB-YP365379ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌seqA重組蛋白
  • 貨號：
    CSB-EP365379ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌seqA重組蛋白
  • 貨號：
    CSB-EP365379ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌seqA重組蛋白
  • 貨號：
    CSB-BP365379ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌seqA重組蛋白
  • 貨號：
    CSB-MP365379ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    seqA
  • Uniprot No.：
  • 別名：
    seqA; b0687; JW0674; Negative modulator of initiation of replication
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-181
  • 氨基酸序列
    MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSAASQ PAAPVTKEVR VASPAIVEAK PVKTIKDKVR AMRELLLSDE YAEQKRAVNR FMLLLSTLYS LDAQAFAEAT ESLHGRTRVY FAADEQTLLK NGNQTKPKHV PGTPYWVITN TNTGRKCSMI EHIMQSMQFP AELIEKVCGT I
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Negative regulator of replication initiation, which contributes to regulation of DNA replication and ensures that replication initiation occurs exactly once per chromosome per cell cycle. Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re-initiation at newly replicated origins. Repression is relieved when the region becomes fully methylated. Can also bind to hemimethylated GATC sequences outside of oriC region. Binds, with less affinity, to fully methylated GATC sites and affects timing of replication. May play a role in chromosome organization and gene regulation.
  • 基因功能參考文獻：
    1. Data indicate that the SeqA protein is localized at midcell for most of the D-period, but colocalizes with the origin region before cell division and initiation of replication. PMID: 25722374
    2. Data suggest that, in the absence of SeqA, the sister-chromatid cohesion 'safety spacer' is destabilized and completely disappears if the replication fork is inhibited. PMID: 24806348
    3. We propose that SeqA binding results in loose inter-duplex junctions that are resistant to Topo IV cleavage PMID: 23990792
    4. Cell cycle analysis showed that loss of SeqA from the old replication forks did not occur at initiation of the new forks, but instead occurs at a time point coinciding with the end of SeqA-dependent origin sequestration. PMID: 22373925
    5. The number of SeqA foci is not directly governed by the number of replication forks, and supports the idea that new DNA may be 'captured' by existing SeqA structures. PMID: 19371375
    6. well-defined SeqA structures are not found in situations of extensive replication fork repair PMID: 19898675
    7. SeqA protein bound to fully methylated, covalently closed or nicked circular DNA generates positive supercoils in vitro in the presence of topoisomerase I or ligase respectively. PMID: 15458410
    8. These results indicate that SeqA binds hemimethylated nascent DNA segments according to the proceeding of replication forks in the chromosome PMID: 15612935
    9. Data report the crystal structure of the oligomerization domain of SeqA (SeqA-N). PMID: 15933720
    10. Overproduction of SeqA4 led to partially restored initiation synchrony, indicating that origin sequestration may not depend on efficient higher-order multimerization into foci, but do require a high local concentration of SeqA. PMID: 16236133
    11. These results suggest that for survival of replication fork damage, SeqA's repression of replication initiation is more important than its effects on nucleoid organization. PMID: 16556234
    12. it has been demonstrated that two GATC sequences, located 82 and 105 bp downstream of the bacteriophage lambda p(R) transcription start site, are necessary for transcription stimulation by SeqA both in vivo and in vitro PMID: 17005979
    13. investigation of how resetting of oriC to the ORC-like complex is coordinated with SeqA-mediated sequestration; during the sequestration period, SeqA repressed pre-RC assembly while ensuring resetting of E. coli ORC PMID: 17114060
    14. These results indicate that unlike oriC, SeqA modulates lambda DNA replication indirectly, most probably by influencing the stability of the lambda replication complex and the transcriptional activation of ori lambda. PMID: 17464080
    15. An examination of the molecular model of Escherichia coli SeqA protein, a negative regulator of chromosome DNA replication which prevents the overinitiation of replication. PMID: 18849124
    16. seqA mutants have increased LPS phosphorylation and exhibit high levels of chromosomal fragmentation. PMID: 19432803

    顯示更多

    收起更多

  • 亞細胞定位：
    Cytoplasm. Note=Localization is cell cycle-dependent. Localizes at midcell in newborn cells, then migrates in opposite directions toward cell quarter sites and remains tethered there until the cell divides.
  • 蛋白家族：
    SeqA family
  • 數據庫鏈接：


主站蜘蛛池模板： 亚洲中文字幕无码人在线| 国产婷婷色综合av蜜臀av| 99久久久无码国产精品性| 人牛交vide欧美xxxx| 99久久国产亚洲高清观看| 欧美做受又硬又粗又大视频| 亚洲男人的天堂在线播放| 成人免费无遮挡无码黄漫视频| 蜜臀色欲av在线播放国产日韩| 国产手机精品一区二区| 男女一边摸一边做爽爽| 大肉大捧一进一出好爽视频动漫 | 国产精品亚洲аv久久| 亂倫近親相姦中文字幕| 久久人妻无码中文字幕第一| 五月色婷婷丁香无码三级| 国产综合色在线精品| 亚洲色成人网站www永久四虎| 蜜臀av国产一区二区三区| 国产免费久久精品99reswag| 亚洲国产精品一区二区成人片| 黑人大荫蒂高潮视频| 黑人巨大无码中文字幕无码| 国产精品人妻熟女男人的天堂| 激情久久av一区av二区av三区| 国产成人无码精品久久久免费 | 国产亚洲精品俞拍视频| 国产日韩在线时看高清视频 | 亚洲精品国产成人99久久6| 九九久re8在线精品视频| 国产美女裸体无遮挡免费视频| 成人片黄网站色大片免费| 性欧美俄罗斯极品| 717影院理论午夜伦八戒| 天天天天躁天天爱天天碰2018| 免费无码黄动漫在线观看| 亚洲2020天天堂在线观看| 亚洲精品偷拍影视在线观看| 国产精品一品二区三区的使用体验| 亚洲国产成人久久综合下载| 无码人妻一区二区三区免费n鬼逝 好爽好湿好硬好大免费视频 |