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  4. Recombinant Escherichia coli NAD-dependent protein deacylase (cobB)

Recombinant Escherichia coli NAD-dependent protein deacylase (cobB)

  • 中文名稱：
    Recombinant Escherichia coli NAD-dependent protein deacylase(cobB)
  • 貨號：
    CSB-YP304314ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli NAD-dependent protein deacylase(cobB)
  • 貨號：
    CSB-EP304314ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli NAD-dependent protein deacylase(cobB)
  • 貨號：
    CSB-BP304314ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli NAD-dependent protein deacylase(cobB)
  • 貨號：
    CSB-MP304314ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cobB
  • Uniprot No.：
  • 別名：
    cobB; ycfY; b1120; JW1106; NAD-dependent protein deacylase; EC 2.3.1.286; Regulatory protein SIR2 homolog
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of P75960-2
  • 表達區域：
    1-242
  • 氨基酸序列
    MEKPRVLVLT GAGISAESGI RTFRAADGLW EEHRVEDVAT PEGFDRDPEL VQAFYNARRR QLQQPEIQPN AAHLALAKLQ DALGDRFLLV TQNIDNLHER AGNTNVIHMH GELLKVRCSQ SGQVLDWTGD VTPEDKCHCC QFPAPLRPHV VWFGEMPLGM DEIYMALSMA DIFIAIGTSG HVYPAAGFVH EAKLHGAHTV ELNLEPSQVG NEFAEKYYGP ASQVVPEFVE KLLKGLKAGS IA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Activates the enzyme acetyl-CoA synthetase by deacetylating 'Lys-609' in the inactive, acetylated form of the enzyme. May also modulate the activity of other propionyl-adenosine monophosphate (AMP)-forming enzymes.
  • 基因功能參考文獻：
    1. discovered that CobB acts as a lipoamidase; results indicate that CobB has the ability to regulate pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase activity under different growth conditions PMID: 28900027
    2. CobB can reverse acetylation regardless of acetyl donor or acetylation mechanism. PMID: 25417765
    3. CobB is a bifunctional enzyme with lysine desuccinylation and deacetylation activities. PMID: 24176774
    4. CobB play crucial roles in the transcription regulation of the periplasmic stress-responsive promoter cpxP when cells of Escherichia coli grow in the presence of glucose, an environment that induces protein acetylation PMID: 21696463
    5. Results suggest that CobB regulates E. coli chemotaxis by deacetylating CheY. PMID: 20345663

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sirtuin family, Class III subfamily
  • 數據庫鏈接：


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