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Recombinant Escherichia coli Murein hydrolase activator NlpD (nlpD)

In Stock
  • 中文名稱:
    大腸桿菌nlpD重組蛋白
  • 貨號:
    CSB-EP359913ENV
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    nlpD
  • Uniprot No.:
  • 別名:
    nlpD; b2742; JW2712Murein hydrolase activator NlpD
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    53.5kDa
  • 表達區域:
    26-379aa
  • 氨基酸序列
    CSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    This recombinant E.coli K12 nlpD protein is an E.coli-expressed protein (Full Length of Mature Protein). At the theoretical level, the steps needed for obtaining a recombinant protein are pretty straightforward. Obtain the nlpD gene, clone it in an expression vector, transform it into the host of choice, induce it, and then, the protein is ready for purification and characterization. In practice, however, dozens of things can go wrong. Poor growth of the host, inclusion body formation, protein inactivity, and even not obtaining any protein at all are some of the problems often found down the pipeline. So we choose the most suitable strain and vector for this recombinant nlpD protein expression and adopt the most optimized expression condition and purification method. Finally, mild condition and subsequent cumbersome protein-refolding procedures ensure this nlpD protein is soluble.

    nlpD is a protein encoding gene that provides an instruction in making a protein named murein hydrolase activator NlpD. The protein encoded by this gene belongs to E.coli NlpD/Haemophilus LppB family. It is similar in sequence to the LppB lipoprotein outer membrane antigen of Haemophilus somnus in Escherichia coli. This protein has metalloendopeptidase activity and is involved in multiple biological processes, including cell cycle, positive regulation of hydrolase activity, response to drug and septum digestion after cytokinesis.

  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
  • 基因功能參考文獻:
    1. Structure-function analysis revealed that the outer membrane localization of NlpD is critical for regulating its amidase activation activity. PMID: 28708841
    2. The authors report that AmiB and EnvC are recruited to division sites independently of one another. The same is also true for AmiC and NlpD. PMID: 21764913
  • 亞細胞定位:
    Cell inner membrane; Lipid-anchor.
  • 蛋白家族:
    E.coli NlpD/Haemophilus LppB family
  • 數據庫鏈接:


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