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  4. Recombinant Escherichia coli Macrolide-specific efflux protein macA (macA)

Recombinant Escherichia coli Macrolide-specific efflux protein macA (macA)

  • 中文名稱：
    大腸桿菌macA重組蛋白
  • 貨號(hào)：
    CSB-YP302788ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌macA重組蛋白
  • 貨號(hào)：
    CSB-EP302788ENV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌macA重組蛋白
  • 貨號(hào)：
    CSB-EP302788ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌macA重組蛋白
  • 貨號(hào)：
    CSB-BP302788ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌macA重組蛋白
  • 貨號(hào)：
    CSB-MP302788ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    macA
  • Uniprot No.：
  • 別名：
    macA; ybjY; b0878; JW0862; Macrolide export protein MacA
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    32-371
  • 氨基酸序列
    PVPTYQTLI VRPGDLQQSV LATGKLDALR KVDVGAQVSG QLKTLSVAIG DKVKKDQLLG VIDPEQAENQ IKEVEATLME LRAQRQQAEA ELKLARVTYS RQQRLAQTKA VSQQDLDTAA TEMAVKQAQI GTIDAQIKRN QASLDTAKTN LDYTRIVAPM AGEVTQITTL QGQTVIAAQQ APNILTLADM SAMLVKAQVS EADVIHLKPG QKAWFTVLGD PLTRYEGQIK DVLPTPEKVN DAIFYYARFE VPNPNGLLRL DMTAQVHIQL TDVKNVLTIP LSALGDPVGD NRYKVKLLRN GETREREVTI GARNDTDVEI VKGLEAGDEV VIGEAKPGAA Q
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.
  • 基因功能參考文獻(xiàn)：
    1. Protoporphyrin (PPIX) efflux by the MacAB-TolC pump. PMID: 25257218
    2. Mutational and biochemical analyses revealed that the conserved residues located at the tip region of the alpha-hairpin of MacA play an essential role in the binding of TolC. PMID: 20307498
  • 亞細(xì)胞定位：
    Cell inner membrane; Single-pass membrane protein; Periplasmic side.
  • 蛋白家族：
    Membrane fusion protein (MFP) (TC 8.A.1) family
  • 數(shù)據(jù)庫(kù)鏈接：


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