在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli Large-conductance mechanosensitive channel (mscL), partial

  • 中文名稱:
    大腸桿菌mscL重組蛋白
  • 貨號:
    CSB-YP358918ENV1
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大腸桿菌mscL重組蛋白
  • 貨號:
    CSB-EP358918ENV1
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大腸桿菌mscL重組蛋白
  • 貨號:
    CSB-EP358918ENV1-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大腸桿菌mscL重組蛋白
  • 貨號:
    CSB-BP358918ENV1
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大腸桿菌mscL重組蛋白
  • 貨號:
    CSB-MP358918ENV1
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    mscL
  • Uniprot No.:
  • 別名:
    mscL; yhdC; b3291; JW3252; Large-conductance mechanosensitive channel
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. Participates in the regulation of osmotic pressure changes within the cell. Opens at a pressure just below that which would cause cell disruption and death. The force required to trigger channel opening depends on the membrane lipids composition.
  • 基因功能參考文獻:
    1. Because MscL opens to tension in the lipid bilayer, requiring no other proteins or ligands, it could be developed into a general noninvasive sonogenetic tool to manipulate the activities of neurons or other cells and potential nanodevices. PMID: 29916253
    2. the amphipathic N-terminal helix of MscL acts as a crucial structural element during tension-induced gating, both stabilizing the closed state and coupling the channel to the membrane PMID: 27329693
    3. More importantly, the FE model indicates that to fully operate an MscL nanovalve by pulling directly on the N-terminus and bottom of TM1, gain-of-function (GOF) mutants (e.g., G22N MscL) would have to be employed rather than the WT MscL channel. PMID: 28859093
    4. Data suggest that dihydrostreptomycin binds to a specific site on mechanosensitive channel of large conductance (MscL) and modifies its conformation, thus allowing the passage of K+ and glutamate out of, and dihydrostreptomycin into, the cell. PMID: 27280286
    5. comprehensive screen has identified many residues that are critical for channel function as well as highlighted MscL domains and residues that undergo the most drastic environmental changes upon gating. PMID: 26368283
    6. Observe directly that the MscL channel opens via the helix-tilt model and the open pore reaches 2.8 nm in diameter. PMID: 24550255
    7. The crystal structure of the cytoplasmic domain of MscL contains canonical hydrophobic and atypical ionic interactions compared to previously characterized coiled coil structures. PMID: 24038743
    8. Results demonstrate the existence of oligomeric forms of MscL not yet observed by X-ray crystallography or other techniques and that in some cases a single type of MscL subunit can assemble as a mixture of oligomeric states. PMID: 21280123
    9. Data show that by using planar membrane bilayers MscL forms functional ion channels capable of ion transport. PMID: 21134371
    10. MscL does not need the insertase YidC for insertion in vitro PMID: 20888317
    11. Data show that introduction of a charge into pore lumen of MscL does indeed influence the permeation of charged molecules. PMID: 20930114
    12. the distribution of channels among the subconducting states of MscL is significantly less dependent on tension than the distribution between the closed and conducting states PMID: 15111402
    13. lipid effects altered activity through examined the contribution of specific headgroups by measuring their effect on the membrane tension required to gate the MscL channel PMID: 16142922
    14. Tyrosines and tryptophans that anchor both ends of the helices to membrane interfaces in many transmembrane proteins are not common in MscL and homologous mechanosensitive channels. PMID: 16156671
    15. YidC is essential for efficient membrane insertion of the MscL protein PMID: 17113597
    16. Pharmacological potential of MscL may involve discovery of new age antibiotics to combat multiple drug-resistant bacterial strains. PMID: 17350877
    17. model for structural transitions and residue-residue proximities that occur upon MscL gating. PMID: 19261722

    顯示更多

    收起更多

  • 亞細胞定位:
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族:
    MscL family
  • 數據庫鏈接:


主站蜘蛛池模板: 国产亚洲精品美女久久久久| 久久精品aⅴ无码中文字字幕| 日本极品少妇videossexhd| 欧美日韩在线第一页免费观看| 久久99国产只有精品| 午夜福制92视频| 热99re久久精品这里都是精品免费 | 免费无码成人av片在线在线播放| 蜜臀av性久久久久蜜臀aⅴ麻豆 | 精品久久久久久无码人妻| 精品久久欧美熟妇www| 欧美黑人巨大xxxxx视频| 亚洲欧洲中文日韩久久av乱码| 国产精品成人免费一区久久羞羞| 窝窝午夜看片成人精品| 一边啪啪一边呻吟av夜夜嗨| 人妻无码人妻有码中文字幕| 国产一区二区精品久久岳| 99精品产国品一二三产区| 久9热视频这里只精品18| 国产乱人激情h在线观看| 亚洲欧美日韩在线码| 日本亚欧热亚洲乱色视频| 色噜噜狠狠狠狠色综合久一| 少妇白浆高潮无码免费区| 国内午夜国产精品小视频| 久久精品aⅴ无码中文字字幕| 人妻丰满被色诱中文字幕| 亚洲欧美日韩成人一区二区三区| 国产成+人+综合+亚洲欧美| 欧美极品少妇无套实战| 白嫩日本少妇做爰| 亚洲国产欧美在线看片一国产| 欧美牲交a欧美牲交aⅴ| 对白脏话肉麻粗话av| 国产成人卡2卡3卡4乱码| 午夜亚洲国产理论片二级港台二级| 俺去俺来也在线www色官网| 最新系列国产专区|亚洲国产| 久久超碰极品视觉盛宴| 欧美日韩一区二区综合|