在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli Holliday junction ATP-dependent DNA helicase RuvA (ruvA)

In Stock
  • 中文名稱:
    大腸桿菌ruvA重組蛋白
  • 貨號(hào):
    CSB-EP359062ENV
  • 規(guī)格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    ruvA
  • Uniprot No.:
  • 別名:
    ruvA; b1861; JW1850; Holliday junction ATP-dependent DNA helicase RuvA; EC 3.6.4.12
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    38.1kDa
  • 表達(dá)區(qū)域:
    1-203aa
  • 氨基酸序列
    MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQEAIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.
  • 基因功能參考文獻(xiàn):
    1. Regression of replication forks stalled by leading-strand template damage: both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially. PMID: 25138216
    2. These results suggest that RecQ acts upstream of RuvABC, RecG and XerC proteins, a finding that is compatible with its primary role in initiation of the RecF recombination pathway. PMID: 24036154
    3. RNA polymerase mutations reduce the synergism between priB and ruvABC. PMID: 22957744
    4. DNA fragmentation absolutely depends on both RecA-catalyzed homologous strand exchange and RuvABC-catalyzed Holliday junction resolution PMID: 22194615
    5. Role in converting the initial single-strand DNA-protein cleavage complex into a double-strand break prior to repair by homologous recombination. PMID: 20601468
    6. Study hypothesized that RuvAB catalyzes replication fork reversal in order to produce SOS(Con) expression. PMID: 20304994
    7. RuvA octamerization is essential for the full biological activity of RuvABC. PMID: 15556943
    8. RuvA is less mobile at a heterologous junction compared to a homologous junction, as two opposing DnaB pumps are required to mobilize RuvA over heterologous DNA. PMID: 16324713
    9. RuvABC is required to resolve junctions that arise during the repair of a subset of nonarresting lesions after replication has passed through the template PMID: 16895921
    10. The authors present here the isolation and characterization of ruvA and ruvB single mutants that are impaired for replication fork reversal at forks arrested by the inactivation of polymerase III, while they remain capable of homologous recombination. PMID: 18942176

    顯示更多

    收起更多

  • 亞細(xì)胞定位:
    Cytoplasm. Note=In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours.
  • 蛋白家族:
    RuvA family
  • 數(shù)據(jù)庫鏈接:


主站蜘蛛池模板: 韩国 日本 亚洲 国产 不卡| 人妻少妇邻居少妇好多水在线 | 四库影院永久四虎精品国产| 亚洲人成网7777777国产| 亚洲成av人在线视| 18禁亚洲深夜福利人口| 日产2021免费一区| 麻豆视传媒在线观看| 日本乱子人伦在线视频| 成年日韩片av在线网站| 青青草国产精品人人爱| 一个人看的免费视频www中文字幕| 亚洲狠狠婷婷综合久久蜜芽| 久久av无码aⅴ高潮av喷吹| 四虎国产精品永久地址49| 欧洲成人午夜精品无码区久久 | 国产国拍亚洲精品av| 熟妇人妻无乱码中文字幕| 久久丫精品国产| 久青草国产97香蕉在线影院| 欧美精品18videosex性欧 | 亚洲人成网站18禁止无码| 亚洲成色www久久网站| 人妻人人澡人人添人人爽| 乱人伦无码中文视频在线| 成人欧美一区二区三区视频| 乱子伦视频在线看| 亚洲乱亚洲乱少妇无码| 中文av伊人av无码av狼人| 欧美无人区码suv| 女人与拘性猛交视频| 无码av动漫精品一区二区免费| 亚洲熟女av乱码在线观看漫画| 国精无码欧精品亚洲一区| 性xxxxx大片免费视频| 亚洲欧美v国产一区二区| 麻豆成人精品国产免费| 99精品久久久久久久婷婷| 在线无码免费的毛片视频| 波多野结衣乳巨码无在线| 欧妇女乱妇女乱视频|