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  4. Recombinant Escherichia coli Hemolysin expression-modulating protein Hha (hha)

Recombinant Escherichia coli Hemolysin expression-modulating protein Hha (hha)

  • 中文名稱：
    大腸桿菌hha重組蛋白
  • 貨號：
    CSB-YP359757ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌hha重組蛋白
  • 貨號：
    CSB-EP359757ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌hha重組蛋白
  • 貨號：
    CSB-EP359757ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌hha重組蛋白
  • 貨號：
    CSB-BP359757ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌hha重組蛋白
  • 貨號：
    CSB-MP359757ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    hha
  • Uniprot No.：
  • 別名：
    hha; b0460; JW0449Hemolysin expression-modulating protein Hha
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-72
  • 氨基酸序列
    MSEKPLTKTD YLMRLRRCQT IDTLERVIEK NKYELSDNEL AVFYSAADHR LAELTMNKLY DKIPSSVWKF IR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Down-regulates hemolysin (hly) expression in complex with H-NS. Stimulates transposition events in vivo. Modifies the set of genes regulated by H-NS; Hha and Cnu (YdgT) increase the number of genes DNA bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex. Binds DNA and influences DNA topology in response to environmental stimuli; does not however interact with DNA in the absence of H-NS. Involved in persister cell formation, acting downstream of mRNA interferase (toxin) MqsR. Decreases biofilm formation by repressing the transcription of fimbrial genes fimA and ihfA, and by repressing the transcription of tRNAs corresponding to rare codons, which are abundant in type 1 fimbrial genes.
  • 基因功能參考文獻：
    1. TomB antitoxin activity is oxygen-dependent and its Yersinia orthologue, YmoB can replace TomB as the Hha antitoxin. PMID: 27929062
    2. The results demonstrated that the chromosomal binding of Hha requires H-NS/StpA, and is necessary for the repression of a subset of genes in the H-NS/StpA regulon. PMID: 23543115
    3. In conclusion, Hha regulates biofilm formation in enterohemorrhagic Escherichia coli O157:H7 by differential regulation of FlhDC and CsgD, the global regulators of motility and curli production, respectively. PMID: 23377937
    4. Authors provide evidence that, as has been shown for H-NS, Hha-like proteins play an essential role facilitating acquisition of horizontally transferred DNA in both Escherichia coli and Salmonella. PMID: 23258263
    5. Hha has to undergo a conformational change to bind H-NS. PMID: 21600204
    6. role of the nucleoid associated proteins H-NS and Hha in the repression of htrA expression at low osmolarity. PMID: 16143461
    7. A single mutation of cysteine 18 to isoleucine (C18I) in Hha abolishes the repression of the hemolysin operon observed in the wild-type protein. PMID: 18675805

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  • 蛋白家族：
    Hha/YmoA/Cnu family
  • 數據庫鏈接：


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