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  4. Recombinant Escherichia coli HTH-type transcriptional regulator malT (malT), partial

Recombinant Escherichia coli HTH-type transcriptional regulator malT (malT), partial

  • 中文名稱：
    大腸桿菌malT重組蛋白
  • 貨號：
    CSB-YP362010ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌malT重組蛋白
  • 貨號：
    CSB-EP362010ENV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌malT重組蛋白
  • 貨號：
    CSB-EP362010ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌malT重組蛋白
  • 貨號：
    CSB-BP362010ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌malT重組蛋白
  • 貨號：
    CSB-MP362010ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    malT
  • Uniprot No.：
  • 別名：
    malT; malA; b3418; JW3381HTH-type transcriptional regulator MalT; ATP-dependent transcriptional activator MalT
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Positively regulates the transcription of the maltose regulon whose gene products are responsible for uptake and catabolism of malto-oligosaccharides. Specifically binds to the promoter region of its target genes, recognizing a short DNA motif called the MalT box (5'-GGA[TG]GA-3'). Displays weak ATPase activity, but this activity is not required for promoter binding.
  • 基因功能參考文獻：
    1. pinpointed the NOD-arm interface of the MalT STAND transcription factor, and generated a MalT variant in which this interface can be covalently locked on demand, thereby trapping the NOD in the closed state PMID: 25740650
    2. The molecular entity that interacts with MalT is not free MalK, but the maltose transporter, MalFGK(2) , which sequesters MalT to the membrane. PMID: 22715926
    3. The inducer maltotriose binds in the central cavity of the tetratricopeptide-like sensor domain of MalT. PMID: 20545845
    4. Substitutions of R171 impaired both ATP hydrolysis and multimerization, which is consistent with an arginine finger function and provides further evidence that ATP hydrolysis is primarily catalyzed by MalT multimers. PMID: 20693326
    5. Data suggest that the MalT/MalK interaction might involve two distinct contact sites on each partner. PMID: 15733913
    6. ATPase activity of malT is not essential for downstream signaling but plays a key role in the integration of regulatory signals. PMID: 17964259
    7. Overexpression of glk leads to a reduction in mal gene expression and interpret of this repression is a direct interaction of Glk with MalT concomitant with MalT inhibition. PMID: 19028900

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  • 蛋白家族：
    MalT family
  • 數(shù)據(jù)庫鏈接：


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