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  4. Recombinant Escherichia coli H (+)/Cl (-) exchange transporter ClcA, partial

Recombinant Escherichia coli H (+)/Cl (-) exchange transporter ClcA, partial

  • 中文名稱：
    Recombinant Escherichia coli H(+)/Cl(-) exchange transporter ClcA,partial
  • 貨號：
    CSB-YP336249ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli H(+)/Cl(-) exchange transporter ClcA,partial
  • 貨號：
    CSB-EP336249ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli H(+)/Cl(-) exchange transporter ClcA,partial
  • 貨號：
    CSB-EP336249ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli H(+)/Cl(-) exchange transporter ClcA,partial
  • 貨號：
    CSB-BP336249ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli H(+)/Cl(-) exchange transporter ClcA,partial
  • 貨號：
    CSB-MP336249ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    clcA
  • Uniprot No.：
  • 別名：
    clcA; eriC; yadQ; b0155; JW5012H(+)/Cl(-) exchange transporter ClcA; ClC-ec1
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.
  • 基因功能參考文獻：
    1. Thermodynamic analysis of ion and proton binding shows how the structure of the CLC transporter effects the pH-independent, 2 Cl- to 1 proton binding stoichiometry. The key features are a helix cage, which creates a compact region with a very strong electropositive potential that stabilizes two anions and a single Glutamate that competes with two Cl ions. PMID: 28877486
    2. Crystallographic study on the ion-binding properties of the ClC selectivity filter in the close homolog from Escherichia coli was presented. PMID: 16341087
    3. The unitary Cl(-) transport rate, measured in liposomes by both initial-velocity method and a novel Poisson dilution approach, is approximately 4,000 s(-1) for wild-type protein, and the uncoupled mutants transport Cl(-) at similar rates PMID: 17389248
    4. E203 substitution with Cys and crystal structures of mutants showed that respective Glu is the internal proton-transfer residue that delivers protons from intracellular solution to the protein interior PMID: 19139174
    5. The authors propose that CLC-ec1 operates through an affinity-switch mechanism and that the bases of substrate specificity are conserved in the CLC channels and transporters. PMID: 19898476

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  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Chloride channel (TC 2.A.49) family, ClcA subfamily
  • 數據庫鏈接：


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