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  4. Recombinant Escherichia coli Glycerol uptake facilitator protein (glpF), partial

Recombinant Escherichia coli Glycerol uptake facilitator protein (glpF), partial

  • 中文名稱：
    大腸桿菌glpF重組蛋白
  • 貨號(hào)：
    CSB-YP365183ENV1
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌glpF重組蛋白
  • 貨號(hào)：
    CSB-EP365183ENV1
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌glpF重組蛋白
  • 貨號(hào)：
    CSB-EP365183ENV1-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌glpF重組蛋白
  • 貨號(hào)：
    CSB-BP365183ENV1
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌glpF重組蛋白
  • 貨號(hào)：
    CSB-MP365183ENV1
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    glpF
  • Uniprot No.：
  • 別名：
    glpF; b3927; JW3898; Glycerol uptake facilitator protein; Aquaglyceroporin
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
  • 基因功能參考文獻(xiàn)：
    1. Mutation of tryptophan Trp219 affects the aqualgyeroporin GlpF activity and the stability of the active GlpF tetramer. PMID: 29069569
    2. Osmotic water permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations have been presented. PMID: 28455098
    3. Folding of the aquaglyceroporin GlpF determines its stability. (Review) PMID: 25462169
    4. TMAO increases the heat stability of the GF tetramer an average of 10 degrees C in the 4 detergents and also protects the protein from denaturation by SDS. PMID: 25387032
    5. The tetrameric alpha-helical GlpF unfolds via a dimeric folding intermediate. PMID: 22035256
    6. GlpF is found to be highly permeable for small solutes and permeable for larger solutes. PMID: 18202181
    7. GlpF exists as a stably folded tetramer in detergent solutions of neutral dodecyl maltoside and in zwitterionic lysomyristoylphosphatidylcholine. PMID: 18284214

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  • 亞細(xì)胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family
  • 數(shù)據(jù)庫鏈接：


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