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  4. Recombinant Escherichia coli G/U mismatch-specific DNA glycosylase (mug)

Recombinant Escherichia coli G/U mismatch-specific DNA glycosylase (mug)

  • 中文名稱(chēng)：
    大腸桿菌mug重組蛋白
  • 貨號(hào)：
    CSB-YP359297ENV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    大腸桿菌mug重組蛋白
  • 貨號(hào)：
    CSB-EP359297ENV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    大腸桿菌mug重組蛋白
  • 貨號(hào)：
    CSB-EP359297ENV-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    大腸桿菌mug重組蛋白
  • 貨號(hào)：
    CSB-BP359297ENV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    大腸桿菌mug重組蛋白
  • 貨號(hào)：
    CSB-MP359297ENV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mug
  • Uniprot No.：
  • 別名：
    mug; ygjF; b3068; JW3040; G/U mismatch-specific DNA glycosylase; EC 3.2.2.28; Double-strand-specific uracil glycosylase; Mismatch-specific uracil DNA-glycosylase; MUG
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-168
  • 氨基酸序列
    MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGR
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.
  • 基因功能參考文獻(xiàn)：
    1. E. coli MUG is able to excise 8oxoA in 8oxoA*T pairs. PMID: 23209024
    2. Mug glycosylase is engaged in the repair of Etheno-DNA adducts. PMID: 21193516
    3. Mug binds its product cooperatively, and a stoichiometric analysis of DNA binding, catalytic activity and salt-dependence indicates that dimer formation is of functional significance in both catalytic activity and product binding. PMID: 21112870
    4. Escherichia coli mismatch-specific uracil DNA glycosylase is a robust xanthine DNA glycosylase PMID: 20852254
    5. The enzymatic activity of E. coli mismatch-specific uracil-DNA glycosylase (MUG)can be complemented by human thymine-DNA glycosylase (hTDG). PMID: 15474419

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Uracil-DNA glycosylase (UDG) superfamily, TDG/mug family
  • 數(shù)據(jù)庫(kù)鏈接：


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