在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli Formamidopyrimidine-DNA glycosylase (mutM)

In Stock
  • 中文名稱:
    Recombinant Escherichia coli Formamidopyrimidine-DNA glycosylase (mutM)
  • 貨號:
    CSB-EP361544ENV
  • 規格:
    ¥1536
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    mutM
  • Uniprot No.:
  • 別名:
    Fapy-DNA glycosylase;DNA-apurinic or apyrimidinic site;AP lyase MutM
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    37.2 kDa
  • 表達區域:
    1-269aa
  • 氨基酸序列
    MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    C-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG) and its derivatives such as guanidinohydantoin:C and spiroiminodihydantoin:C, however it also acts on thymine glycol:G, 5,6-dihydrouracil:G and 5-hydroxyuracil:G. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Cleaves ssDNA containing an AP site.
  • 基因功能參考文獻:
    1. expression of the bacterial DNA repair protein FPG stably protects human lung cells from the mutagenic effects of CS by improving cells' capacity to repair damaged DNA. PMID: 24498234
    2. Structural and biochemical analysis of DNA helix invasion by the bacterial 8-oxoguanine DNA glycosylase MutM PMID: 23404556
    3. Fpg protein seemed to be very important in the repair of ultraviolet-C induced DNA lesions. PMID: 22732937
    4. structures reveal that MutM imposes the same extrusion-prone backbone conformation on the oxoG lesion irrespective of its 5'-neighbor while leaving the rest of the DNA relatively free to adjust to the particular demands of individual sequences PMID: 22465958
    5. demonstrates that it is feasible to increase resistance of human TM cell to endogenous oxidative damage by FPG gene transfection PMID: 21561236
    6. Over-expressed, purified and characterized two stable isotope-labeled DNA glycosylases, i.e., (15)N-labeled Escherichia coli formamidopyrimidine DNA glycosylase (Fpg) and (15)N-labeled human 8-oxoguanine-DNA glycosylase (hOGG1). PMID: 21356311
    7. examination of the structural and dynamic changes that occur in solution when Fpg binds DNA PMID: 15661656
    8. A base excision repair enzyme involved in the 8-oxoguanine (oxoG) repair pathway. PMID: 15979229
    9. when the enzyme encounters its true substrate, 8-oxoG.C, the complex enters the productive catalytic reaction after approximately 50 ms, partitioning the substrate away from the competing dissociation process PMID: 17209553
    10. The substrate specificity of two similar enzymes, MutM of E. coli and OGG1 of H. sapiens, is reported. PMID: 17536801
    11. The high affinity and efficient activity of EcFpg toward the hydantoin lesions suggest that EcFpg mediates repair of the lesions in vivo. PMID: 17655276
    12. Data show a lack of interaction of mutM with dnaX36 since the double dnaX36 mutM strains did not show any major changes in the frequency of G.CT.A transversions compared to the single dnaX36 strain. PMID: 18156258
    13. analysis of the the driving forces that dictate Fpg enzyme efficiency and specificity, which helps to elucidate the energy landscape for lesion recognition and repair PMID: 18172202
    14. Data show that processive cleavage by Fpg does not correlate with their substrate specificity and under nearly physiological salt conditions may be replaced with the distributive mode of action. PMID: 18672903

    顯示更多

    收起更多

  • 蛋白家族:
    FPG family
  • 數據庫鏈接:


主站蜘蛛池模板: 茄子在线看片免费人成视频| 亚洲日本中文字幕天天更新| 少妇无码av无码专区线| 久久精品成人免费国产片小草 | 99久久国产亚洲高清观看| 精品无码专区亚洲| 性生交大片免费看女人按摩| 中文无码熟妇人妻av在线| 老司机久久一区二区三区| 男女性高爱潮免费观看| 午夜精品一区二区三区免费视频 | 老牛精品亚洲成av人片| 一本久道久久综合久久爱| 精品丝袜国产自在线拍小草| 99久热在线精品996热是什么 | 国产精品亚洲一区二区三区在线| 美女裸体十八禁免费网站| 久久天天躁夜夜躁狠狠85台湾| 久热在线播放中文字幕| 久久综合亚洲色一区二区三区| 岛国片人妻三上悠亚| 77777亚洲午夜久久多喷| 国产精品亚洲а∨无码播放麻豆| 无码国产色欲xxxx视频| 综合三区后入内射国产馆| 娜娜麻豆国产电影| 中文字幕乱码免费| 天天爽天天爽天天爽| 亚洲精品国产成人无码区a片| 亚洲狠狠做深爱婷婷影院| 另类国产精品一区二区| 水蜜桃av无码一区二区| 一区二区乱子伦在线播放 | 精品久久久久久777米琪桃花| 免费女人高潮流视频在线| 国产午夜伦鲁鲁| 人妻少妇69式99偷拍| 华人在线亚洲欧美精品 | 日本欧美一区二区免费视频| 天天爽夜夜爽人人爽免费| 国产乱人伦av在线无码|