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  4. Recombinant Escherichia coli Ferrichrome-iron receptor (fhuA), partial

Recombinant Escherichia coli Ferrichrome-iron receptor (fhuA), partial

  • 中文名稱：
    大腸桿菌fhuA重組蛋白
  • 貨號：
    CSB-YP362005ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌fhuA重組蛋白
  • 貨號：
    CSB-EP362005ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌fhuA重組蛋白
  • 貨號：
    CSB-BP362005ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌fhuA重組蛋白
  • 貨號：
    CSB-MP362005ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    fhuA
  • Uniprot No.：
  • 別名：
    fhuA; tonA; b0150; JW0146Ferrichrome outer membrane transporter/phage receptor; Ferric hydroxamate receptor; Ferric hydroxamate uptake; Ferrichrome-iron receptor
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in the uptake of iron in complex with ferrichrome, a hydroxamate-type siderophore. Binds and transports ferrichrome-iron across the outer membrane. In addition to its role in ferrichrome-iron transport, transports the antibiotic albomycin, which is a structural analog of ferrichrome, and acts as a receptor for colicin M, microcin J25 and bacteriophages T1, T5, phi80 and UC-1. The energy source, which is required for all FhuA functions except infection by phage T5, is provided by the inner membrane TonB system.
  • 基因功能參考文獻：
    1. Cor is an outer membrane (OM) lipoprotein that requires the participation of additional outer membrane proteins (OMPs) to exclude FhuA-dependent phages. PMID: 30043202
    2. FhuA-PNIPAAm conjugates will serve as functional nanosized building blocks for applications in targeted drug delivery, self-assembly systems, functional membranes and transmembrane protein gated nanoreactors. PMID: 27614163
    3. FhuA plug domain was a mostly unfolded structure that was able to occlude isolated FhuA beta-barrels inserted into the cell membrane. PMID: 22846061
    4. In vitro experiments establish that FhuA-TonB interactions require sequential steps of kinetically limiting rearrangements. PMID: 15736954
    5. during siderophore transport through FhuA, the cork domain remains within the barrel rather than being displaced PMID: 15994322
    6. Results identify specific molecular contacts between TonB and outer membrane receptors such as FhuA that extend beyond the well-characterized Ton box. PMID: 16414071
    7. the 3.3 angstrom resolution crystal structure of the TonB carboxyl-terminal domain in complex with FhuA PMID: 16741125
    8. TonB binding promotes conformational changes in outer surface-exposed loops of FhuA. PMID: 18653801

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  • 亞細胞定位：
    Cell outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    TonB-dependent receptor family
  • 數據庫鏈接：


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