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Recombinant Escherichia coli Exopolyphosphatase (ppx)

In Stock
  • 中文名稱:
    大腸桿菌ppx重組蛋白
  • 貨號:
    CSB-EP365319ENV
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP365319ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) ppx.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP365319ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) ppx.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    ppx
  • Uniprot No.:
  • 別名:
    ppx; b2502; JW2487; Exopolyphosphatase; ExopolyPase; EC 3.6.1.11; Metaphosphatase
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    74.0kDa
  • 表達區域:
    2-513aa
  • 氨基酸序列
    PIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQARRVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNSDLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEESTPEIAA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    The DNA fragment encoding the 2-513aa of the E.coli ppx protein was fused with N-terminal 6xHis-SUMO tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant E.coli ppx protein. The purity of this recombinant ppx protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This recombinant ppx protein showed a band on the gel with a molecular weight of approximately 68-91 kDa.

    ppx is a gene encoding a protein named Exopolyphosphatase and belongs GppA/Ppx family. Exopolyphosphatase (PPX),a phosphatase enzyme, was discovered by the lab of Nobel laureate Arthur Kornberg in 1993.The structure of PPX is characterized by the actin-like ATPase domain. This protein catalyzes and releases orthophosphate processively from the ends of the polyP chain which is a polymer composed of a few to hundreds of phosphate residues supporting cell survival in the stationary phase. This enzyme plays important role in the bacterial stringent response.

  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Degradation of inorganic polyphosphates (polyP). Releases orthophosphate processively from the ends of the polyP chain. Has a strong preference for long-chain polyphosphates and has only weak affinity for smaller size polyP of about 15 residues.
  • 基因功能參考文獻:
    1. H(378) is a fundamental gatekeeper for the recognition of long chain polyphosphate PMID: 27424154
  • 亞細胞定位:
    Cell membrane; Peripheral membrane protein.
  • 蛋白家族:
    GppA/Ppx family
  • 數據庫鏈接:


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