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Recombinant Escherichia coli Exodeoxyribonuclease III (xthA)

  • 中文名稱:
    大腸桿菌xthA重組蛋白
  • 貨號:
    CSB-YP362578ENV
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大腸桿菌xthA重組蛋白
  • 貨號:
    CSB-EP362578ENV
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大腸桿菌xthA重組蛋白
  • 貨號:
    CSB-EP362578ENV-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大腸桿菌xthA重組蛋白
  • 貨號:
    CSB-BP362578ENV
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大腸桿菌xthA重組蛋白
  • 貨號:
    CSB-MP362578ENV
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    xthA
  • Uniprot No.:
  • 別名:
    xthA; xth; b1749; JW1738Exodeoxyribonuclease III; EXO III; Exonuclease III; EC 3.1.11.2; AP endonuclease VI
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-268
  • 氨基酸序列
    MKFVSFNING LRARPHQLEA IVEKHQPDVI GLQETKVHDD MFPLEEVAKL GYNVFYHGQK GHYGVALLTK ETPIAVRRGF PGDDEEAQRR IIMAEIPSLL GNVTVINGYF PQGESRDHPI KFPAKAQFYQ NLQNYLETEL KRDNPVLIMG DMNISPTDLD IGIGEENRKR WLRTGKCSFL PEEREWMDRL MSWGLVDTFR HANPQTADRF SWFDYRSKGF DDNRGLRIDL LLASQPLAEC CVETGIDYEI RSMEKPSDHA PVWATFRR
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities.
  • 基因功能參考文獻:
    1. This increased MMS sensitivity was not suppressed by the presence of multi-copy holC. Addition of the yoaA disruption increased the methyl methanesulfonate (MMS) sensitivity of xthA single-deletion or xthA nfo double-deletion mutants. PMID: 27150717
    2. Xth may act in a complementary repair pathway to nucleotide excision repair to remove S-cdA adducts from 3' DNA termini. PMID: 23898172
    3. Endonuclease IV and Exonuclease III have roles in counteracting DNA damage induced by the oxidative counterpart of UVB in E.coli PMID: 16524897
    4. It is shown (paradoxically) that in xthA mutants, RecA-GFP loading is predominantly RecBCD-dependent and that xthA recB double mutants are viable. PMID: 18034795
  • 蛋白家族:
    DNA repair enzymes AP/ExoA family
  • 數據庫鏈接:


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