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  4. Recombinant Escherichia coli Ditrans,polycis-undecaprenyl-diphosphate synthase ( (2E,6E)-farnesyl-diphosphate specific) (ispU)

Recombinant Escherichia coli Ditrans,polycis-undecaprenyl-diphosphate synthase ( (2E,6E)-farnesyl-diphosphate specific) (ispU)

  • 中文名稱：
    大腸桿菌ispU重組蛋白
  • 貨號：
    CSB-YP350241ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌ispU重組蛋白
  • 貨號：
    CSB-EP350241ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌ispU重組蛋白
  • 貨號：
    CSB-BP350241ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌ispU重組蛋白
  • 貨號：
    CSB-MP350241ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ispU
  • Uniprot No.：
  • 別名：
    ispU; rth; uppS; yaeS; b0174; JW0169; Ditrans,polycis-undecaprenyl-diphosphate synthase; (2E,6E)-farnesyl-diphosphate specific; EC 2.5.1.31; Ditrans,polycis-undecaprenylcistransferase; Undecaprenyl diphosphate synthase; UDS; Undecaprenyl pyrophosphate synthase; UPP synthase
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-253
  • 氨基酸序列
    MMLSATQPLS EKLPAHGCRH VAIIMDGNGR WAKKQGKIRA FGHKAGAKSV RRAVSFAANN GIEALTLYAF SSENWNRPAQ EVSALMELFV WALDSEVKSL HRHNVRLRII GDTSRFNSRL QERIRKSEAL TAGNTGLTLN IAANYGGRWD IVQGVRQLAE KVQQGNLQPD QIDEEMLNQH VCMHELAPVD LVIRTGGEHR ISNFLLWQIA YAELYFTDVL WPDFDEQDFE GALNAFANRE RRFGGTEPGD ETA
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Generates ditrans,octacis-undecaprenyl pyrophosphate (UPP) from isopentenyl pyrophosphate (IPP) and farnesyl diphosphate (FPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide.
  • 基因功能參考文獻(xiàn)：
    1. Unlike the sequential mechanism used by trans-prenyltransferases, these data demonstrate E. coli UPPS utilizes the concerted mechanism. PMID: 20828539
    2. L85, L88 or F89 are important for substrate binding but not specificity. The upper portion of the active-site tunnel, where cis double bonds of the product reside, may be critical for determining the final product chain length. PMID: 15447632
    3. undecaprenyl pyrophosphate synthase (UPPs)is crystallized in a new trigonal unit cell with Mg(2+)/isopentenyl pyrophosphates (IPP)/farnesyl thiopyrophosphate bound to the active site PMID: 15788389
  • 蛋白家族：
    UPP synthase family
  • 數(shù)據(jù)庫鏈接：


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