在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Escherichia coli DNA translocase FtsK (ftsK), partial

Recombinant Escherichia coli DNA translocase FtsK (ftsK), partial

  • 中文名稱：
    大腸桿菌ftsK重組蛋白
  • 貨號：
    CSB-YP342837ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌ftsK重組蛋白
  • 貨號：
    CSB-EP342837ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌ftsK重組蛋白
  • 貨號：
    CSB-EP342837ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌ftsK重組蛋白
  • 貨號：
    CSB-BP342837ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌ftsK重組蛋白
  • 貨號：
    CSB-MP342837ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ftsK
  • Uniprot No.：
  • 別名：
    ftsK; b0890; JW0873; DNA translocase FtsK
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif. Stoppage of translocation is accompanied by a reduction in ATPase activity. Also stimulates topoisomerase 4 activity. Required for the targeting of FtsQ, FtsL and FtsI to the septum.
  • 基因功能參考文獻：
    1. FtsKgamma interacts with the C-terminal domain of XerD, above a cleft where XerC is thought to bind. We present a model for activation of recombination based on structural data. PMID: 27708355
    2. We found that whatever the growth conditions, sister copies of the V. cholerae chr1 dif region remain together at mid-cell until the onset of constriction, which permits their processing by FtsK and the activation of dif-recombination. PMID: 28358835
    3. FtsK translocase has a role in assembly, translocation, and activation of XerCD-dif recombination PMID: 26324908
    4. An average of 7 FtsK hexamers per cell are present at midcell, with the N-terminal domain being able to hexamerize independently of the translocase. PMID: 24302254
    5. Kinetic analyses showed that the presence of the FtsK gamma domain increases the rate of XerCD-dif synapse assembly and increases its stability. PMID: 24214995
    6. Data indicate that the arrival of the FtsK translocase at an initial synaptic complex, forming a transient activated complex in which XerD exchanged a pair of strands to form an Holliday junctions (HJ) intermediate for catalysis by XerC. PMID: 24101525
    7. FtsK segregates the terminus region of sister chromosomes whether they are monomeric or dimeric and does so in an accurate and ordered manner. PMID: 23781109
    8. Multiple regions in FtsK protein are required for in cell division. PMID: 21091498
    9. The properties in vivo and in vitro of FtsK and its relatives are discussed in relation to the biological functions of these remarkable enzymes. PMID: 20298190
    10. FtsK transports DNA before membrane fusion, at a time when there is still an opening in the constricted septum. PMID: 20033058
    11. FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. PMID: 19854947
    12. Data describe the in vivo effects of deletions of FtsK(L) and/or of FtsK(C), of swaps of these domains with their Haemophilus influenzae counterparts and of a point mutation that inactivates the walker A motif of FtsK(C). PMID: 15522074
    13. directly observed the movement of purified FtsK on single DNA molecules; results imply that FtsK is a bidirectional motor that changes direction in response to short, asymmetric directing DNA sequences PMID: 15681387
    14. FtsK induces approximately 0.07 supercoils per DNA helical pitch traveled PMID: 15821742
    15. translocation by FtsK occurs mostly on DNA belonging to a specifically organized domain of the chromosome, when physical links between either dimeric or still intercatenated chromosomes force this DNA to run across the septum at division PMID: 15916604
    16. Results suggest that the N-terminal domain of FtsK is normally involved in stability of the division protein machine and shares functional overlap with FtsQ, FtsB, FtsA, ZipA and FtsN. PMID: 16194242
    17. FtsK translocase is a powerful motor that is able to displace a triplex-forming oligo from a DNA substrate PMID: 16301526
    18. A 62-amino-acid fragment of FtsK(C) interacts directly with the XerD C-terminus in order to stimulate the cleavage by XerD of BSN, a dif-DNA suicide substrate containing a nick in the 'bottom' strand. PMID: 16553881
    19. Study provides evidence that 8-base-pair asymmetric sequences (KOPS) promote FtsK loading on DNA and that translocation is oriented at this step. PMID: 17041597
    20. Study uses in vitro single-molecule and ensemble methods to unveil a mechanism of action in which the translocation and sequence-recognition activities are performed by different domains in FtsK. PMID: 17041598
    21. controls recombination provoked by an extra Ter site in the chromosome terminus PMID: 17376083
    22. Septum localization and concentration of FtsK co-ordinate its various roles in chromosome segregation and cell division. PMID: 18363794
    23. FtsK interacts with the cell division partner proteins through different functional domians. PMID: 18759781
    24. FtsK mediates a reciprocal control between processing of the replichore polarity junction and cell division. PMID: 19057667
    25. Failure to recognize KOPS during FtsK translocation has only modest consequences for fitness of wild-type cells. PMID: 19170870
    26. The structure and composition of DNA interferes with Xer recombination activation by FtsK. PMID: 19246541

    顯示更多

    收起更多

  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein. Note=Located at the septum. Colocalizes with FtsZ. ZipA, FtsZ and FtsA, but not FtsI and FtsQ are required to target it to the septum.
  • 蛋白家族：
    FtsK/SpoIIIE/SftA family
  • 數據庫鏈接：


主站蜘蛛池模板： 免费无码又爽又刺激高潮的app| 国产aⅴ精品一区二区三区久久| 国产福利无码一区在线| 国产综合久久亚洲综合| 国产精品理论片| 国模小婕私拍鲜嫩玉门| 久久久久中文伊人久久久| 亚洲伊人成综合网2222| 国产精品老热丝在线观看| 伊人情人色综合网站| 国产主播一区二区三区在线观看| 国产午夜福利伦理300| 看免费真人视频网站| 国产亚洲精品久久久久久小舞| 野外被强j到高潮免费观看| 精品无码久久久久国产| 无码夜色一区二区三区| 国产精品一区二区香蕉| 少妇无码av无码专区线y| 国产精品成人av在线观看| 西西人体44www大胆无码| 蜜乳av久久久久久久久久久| 潮喷大喷水系列无码| 亚洲精品永久在线观看| 国产午夜精品久久精品电影 | 久久久久国产一区二区| 在线观看免费人成视频网| 好爽好硬好深高潮视频456| 人妻无码视频一区二区三区| 正在播放东北夫妻内射| 国产人在线成免费视频| 国产午夜福利视频在线观看| 久热国产vs视频在线观看| 人妻无码久久中文字幕专区| 国产综合久久亚洲综合| …日韩人妻无码精品一专区| 免费看成人午夜福利专区| 狠狠做五月深爱婷婷| 亚洲精品一区二区三区在线| 国产精品女上位好爽在线| 欧洲一卡2卡3卡4卡乱码视频 |