在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli DNA polymerase II (polB)

In Stock
  • 中文名稱:
    大腸桿菌polB重組蛋白
  • 貨號:
    CSB-EP321908ENV
  • 規格:
    ¥1536
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 別名:
    (Pol II)
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    97.4 kDa
  • 表達區域:
    2-783aa
  • 氨基酸序列
    AQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAFIPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVTVYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETTRHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPDVIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIIDGIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLKDCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLGEVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIGRALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEGDKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARLVYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPLDYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Thought to be involved in DNA repair and/or mutagenesis. Its processivity is enhanced by the beta sliding clamp (dnaN) and clamp loader.
  • 基因功能參考文獻:
    1. polB gene expression is controlled by SOS and RpoS regulons. PMID: 28468910
    2. Polymerases II, IV and V may bypass trans-4-hydroxy-2-nonenal modified bases by translesion synthesis and restore DNA synthesis beyond the arrested site, although with different efficiencies. PMID: 22001238
    3. Data report that 15% of stress-induced mutations associated with DNA double-strand break repair in carbon-starved Escherichia coli requires high-fidelity Pol II, and that this component also requires RpoS. PMID: 20639336
    4. transcription by single Pol II complexes allows survival of the original H3/H4 histones, while multiple, closely spaced complexes of transcribing Pol II can induce displacement of all core histones along the gene PMID: 20534568
    5. Compared to the replicative B family polymerases, DNA Pol II has subtle amino acid changes remote from the active site that allow it to replicate normal DNA with high efficiency yet conduct translesion synthesis when needed. PMID: 20064374
    6. The results fit a model in which Pol II acts as proofreader for HE-produced misinsertion errors. PMID: 16164549
    7. These data suggest that DNA polymerase II is responsible for the in vivo mutagenic bypass of these lesions in wild-type E. coli. PMID: 16257273
    8. elevated expression of Pol-II leads to an approximately 100-fold increase in spontaneous mutagenesis in a manner that is independent of SOS, umuDC, dinB, recA, uvrA and mutS functions PMID: 17586534
    9. Data show that an important role is described for DNA Pol II in preventing the mutations and reveal that Pol II is a major editor of Pol III mediated replication errors. PMID: 18156258

    顯示更多

    收起更多

  • 蛋白家族:
    DNA polymerase type-B family
  • 數據庫鏈接:

    KEGG: ecj:JW0059

    STRING: 316407.85674307



主站蜘蛛池模板: 久久久久国色av免费观看| 无码高潮又爽又黄a片日本动漫| 亚洲精品国产自在现线看| 欧美大波少妇在厨房被| 特级aaaaaaaaa毛片免费视频| 国产思思99re99在线观看| 久久精品无码一区二区app| 国产精品va在线观看老妇女| 国产免费观看久久黄av片| 国产99久久久国产精品~~牛| 日韩精品无码一本二本三本| 精品国产av一区二区果冻传媒 | 97色精品视频在线观看| 久久综合噜噜激激的五月天| 国产av无码专区亚洲精品| 中文字幕奈奈美被公侵犯| 四虎www成人影院观看| 97久久超碰亚洲视觉盛宴| 欧洲精品欧美精品| 日本在线a一区视频高清视频 | 午夜亚洲国产理论片中文| 亚洲爆乳少妇无码激情| 精品www日韩熟女人妻| 日韩人妻无码一区二区三区综合 | 国产无遮挡又黄又爽在线视频| 肉体裸交137日本大胆摄影| 无码h黄肉3d动漫在线观看| 亚洲欧洲av综合色无码| 人人爽天天碰狠狠添| 国产精品久久久久久人妻精品 | 未满小14洗澡无码视频网站| 国产伦子系列沙发午睡| 亚洲日韩va在线视频| 亚洲一区二区三区av天堂| 中字幕人妻一区二区三区| 天堂在/线中文在线资源 官网 | 亚洲国产精品久久久久久| 少妇扒开双腿让我看个够| 极品新婚夜少妇真紧| 欧美v亚洲v日韩v最新在线| 中文www天堂|