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  4. Recombinant Escherichia coli DNA polymerase I (polA), partial

Recombinant Escherichia coli DNA polymerase I (polA), partial

  • 中文名稱：
    大腸桿菌polA重組蛋白
  • 貨號：
    CSB-YP360555ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌polA重組蛋白
  • 貨號：
    CSB-EP360555ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌polA重組蛋白
  • 貨號：
    CSB-EP360555ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌polA重組蛋白
  • 貨號：
    CSB-BP360555ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌polA重組蛋白
  • 貨號：
    CSB-MP360555ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    polA
  • Uniprot No.：
  • 別名：
    polA; resA; b3863; JW3835DNA polymerase I; POL I; EC 2.7.7.7
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.
  • 基因功能參考文獻：
    1. Findings indicate an unrealized function of DNA polymerase I (Pol I), and a possible mechanism by which G4 structures can be resolved during DNA replication and/or repair. PMID: 28986969
    2. Pol I catalyzes DNA synthesis past N6-dA-linked peptide cross-links and is likely to play an role in cellular bypass of large major groove DNA lesions. PMID: 21622737
    3. analysis of complexes of DNA polymerase I (Klenow fragment) for base and sugar discrimination during nucleotide substrate selection PMID: 21362617
    4. a new in vivo function for wild-type DNA polymerase I in chromosome termination at site(s) not yet identified is proposed PMID: 15752206
    5. mechanism in which Arg668 forms a hydrogen bonding fork between the minor groove of the primer terminus and the ring oxygen of the deoxyribose moiety of the incoming dNTP to align the 3'-hydroxyl group with the alpha-phosphate of the dNTP PMID: 15823023
    6. Glutamine677 is perhaps the first residue in the pol I family of enzymes that is shown to be essential for the functioning of both polymerase and proofreading activities. PMID: 15924429
    7. mutant(Delta)polA is defective in base mismatch correction PMID: 16005896
    8. analysis of amino acid substitutions that increase the fidelity of Escherichia coli DNA polymerase I PMID: 17301051
    9. Data show that dnaX36 strains have an increased requirement for DNA polymerase I. PMID: 18156258

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  • 蛋白家族：
    DNA polymerase type-A family
  • 數據庫鏈接：


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