在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli DNA adenine methylase (dam)

In Stock
  • 中文名稱:
    Recombinant Escherichia coli DNA adenine methylase(dam)
  • 貨號(hào):
    CSB-EP360101ENVh2
  • 規(guī)格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    dam
  • Uniprot No.:
  • 別名:
    (DNA adenine methyltransferase)(Deoxyadenosyl-methyltransferase)(Orphan methyltransferase M.EcoKDam)(M.EcoKDam)
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    41.4
  • 表達(dá)區(qū)域:
    1-278aa
  • 氨基酸序列
    MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 6xHis-tagged and C-terminal Avi-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.
  • 基因功能參考文獻(xiàn):
    1. Structural analysis of a new Dam binding site on DNA with implications for methylation-independent transcriptional repression. PMID: 25845600
    2. We propose a model for intrasite methylation in which Dam bound to flanking DNA is an obligate intermediate. PMID: 22570478
    3. structural and functional analysis PMID: 15456775
    4. the up-regulation of recombinational repair in dam mutants allows for the efficient repair of double-strand breaks whose formation is dependent on functional mismatch repair PMID: 16199573
    5. DNA regions accessible to Dam methylase in transfected human cells apparently coincided with those hypersensitive to DNase I. PMID: 16897455
    6. Lys(14) is important for EcoDam binding of the extrahelical target base into the active site pocket PMID: 17545164
    7. only at low Lrp concentrations will Dam compete effectively for binding and methylation of the proximal GATC site, leading to a phase switch resulting in the expression of pili PMID: 18706913
    8. Unlike other restriction-modification methyltransferases, E. coli DNA adenine methyltransferase carries out intrasite processive catalysis whereby the enzyme-DNA complex methylates both strands of unmethylated GATC site prior to dissociation from DNA. PMID: 19580332

    顯示更多

    收起更多

  • 蛋白家族:
    N(4)/N(6)-methyltransferase family
  • 數(shù)據(jù)庫鏈接:


主站蜘蛛池模板: 中文字幕无码中文字幕有码a| 午夜性开放午夜性爽爽| 偷欧洲亚洲另类图片av天堂| 国语自产拍在线观看对白| 久久无码高潮喷水| 国产乱码精品一区二区三区中文| 天堂mv在线mv免费mv香蕉| 亚洲欧美综合精品久久成人网| 国产精品女视频一区二区| 成人免费无码大片a毛片抽搐色欲| 国产精品无码一区二区三区 | 久久av无码精品人妻系列试探| 日韩人妻一区二区三区免费 | 天码av无码一区二区三区四区| 国产偷伦视频片免费视频| 国产最新av在线播放不卡| 婷婷综合久久中文字幕| 一本加勒比hezyo无码资源网 | 亚洲色成人一区二区三区小说| 亚洲一区二区制服在线| 亚洲愉拍自拍另类图片| 亚洲日韩精品a∨片无码| 免费无码av片在线观看| 四库影院永久国产精品地址| 精品人妻无码一区二区三区蜜桃一| 成年丰满熟妇午夜免费视频| 国产真实野战在线视频| 国产成人午夜福利电影在线播放| 国产成人亚洲综合| 国产成人免费ā片在线观看| 国产99青草视频在线播放视| av在线无码专区一区| 亚洲日韩乱码久久久久久| 精品国产久九九| 男女做爰无遮挡性视频 | 日韩精品久久久久久免费| 美女露全乳无遮掩视频| 久久久av男人的天堂| 色优久久久久综合网鬼色| 伊人久久久精品区aaa片| 亚洲成av人片不卡无码久久 |