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Recombinant Escherichia coli Cysteine desulfurase (iscS)

  • 中文名稱:
    Recombinant Escherichia coli Cysteine desulfurase(iscS)
  • 貨號:
    CSB-YP015769ENV
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli Cysteine desulfurase(iscS)
  • 貨號:
    CSB-EP015769ENV
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli Cysteine desulfurase(iscS)
  • 貨號:
    CSB-EP015769ENV-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli Cysteine desulfurase(iscS)
  • 貨號:
    CSB-BP015769ENV
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli Cysteine desulfurase(iscS)
  • 貨號:
    CSB-MP015769ENV
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    iscS
  • Uniprot No.:
  • 別名:
    iscS; nuvC; yfhO; yzzO; b2530; JW2514; Cysteine desulfurase IscS; EC 2.8.1.7; NifS protein homolog; ThiI transpersulfidase; TusA transpersulfidase
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    full length protein
  • 表達(dá)區(qū)域:
    1-404
  • 氨基酸序列
    MKLPIYLDYS ATTPVDPRVA EKMMQFMTMD GTFGNPASRS HRFGWQAEEA VDIARNQIAD LVGADPREIV FTSGATESDN LAIKGAANFY QKKGKHIITS KTEHKAVLDT CRQLEREGFE VTYLAPQRNG IIDLKELEAA MRDDTILVSI MHVNNEIGVV QDIAAIGEMC RARGIIYHVD ATQSVGKLPI DLSQLKVDLM SFSGHKIYGP KGIGALYVRR KPRVRIEAQM HGGGHERGMR SGTLPVHQIV GMGEAYRIAK EEMATEMERL RGLRNRLWNG IKDIEEVYLN GDLEHGAPNI LNVSFNYVEG ESLIMALKDL AVSSGSACTS ASLEPSYVLR ALGLNDELAH SSIRFSLGRF TTEEEIDYTI ELVRKSIGRL RDLSPLWEMY KQGVDLNSIE WAHH
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能:
    Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Preferentially binds to disordered IscU on which the Fe-S is assembled, IscU converts to the structured state and then dissociates from IscS to transfer the Fe-S to an acceptor protein. Also functions as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate. Transfers sulfur onto 'Cys-456' of ThiI and onto 'Cys-19' of TusA in transpersulfidation reactions.
  • 基因功能參考文獻(xiàn):
    1. human NFS1 was almost fully able to complement the role of IscS in Moco biosynthesis when its specific interaction partner protein MOCS3 from humans was also present. PMID: 28766335
    2. TusA (YhhP) and IscS are required for molybdenum cofactor-dependent base-analog detoxification. PMID: 23894086
    3. These results provide a clue to the possible role of iscS in pyrimidine metabolism by gene regulation. PMID: 18482579
    4. The desulfurase activity of IscS is gradually inhibited as the amount of iron and sulfide bound to IscS increases. PMID: 18639525
    5. CyaY participates in iron-sulfur (Fe-S) cluster assembly as an iron-dependent inhibitor of cluster formation, through binding to the desulfurase IscS. PMID: 19305405

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  • 亞細(xì)胞定位:
    Cytoplasm.
  • 蛋白家族:
    Class-V pyridoxal-phosphate-dependent aminotransferase family, NifS/IscS subfamily
  • 數(shù)據(jù)庫鏈接:


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