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  4. Recombinant Escherichia coli Cyclopropane-fatty-acyl-phospholipid synthase (cfa)

Recombinant Escherichia coli Cyclopropane-fatty-acyl-phospholipid synthase (cfa)

  • 中文名稱：
    Recombinant Escherichia coli Cyclopropane-fatty-acyl-phospholipid synthase(cfa)
  • 貨號：
    CSB-YP364333ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Cyclopropane-fatty-acyl-phospholipid synthase(cfa)
  • 貨號：
    CSB-EP364333ENV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Cyclopropane-fatty-acyl-phospholipid synthase(cfa)
  • 貨號：
    CSB-EP364333ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Cyclopropane-fatty-acyl-phospholipid synthase(cfa)
  • 貨號：
    CSB-BP364333ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Cyclopropane-fatty-acyl-phospholipid synthase(cfa)
  • 貨號：
    CSB-MP364333ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cfa
  • Uniprot No.：
  • 別名：
    cfa; cdfA; b1661; JW1653Cyclopropane-fatty-acyl-phospholipid synthase; CFA synthase; Cyclopropane fatty acid synthase; EC 2.1.1.79
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    2-382
  • 氨基酸序列
    SSSCIEEVS VPDDNWYRIA NELLSRAGIA INGSAPADIR VKNPDFFKRV LQEGSLGLGE SYMDGWWECD RLDMFFSKVL RAGLENQLPH HFKDTLRIAG ARLFNLQSKK RAWIVGKEHY DLGNDLFSRM LDPFMQYSCA YWKDADNLES AQQAKLKMIC EKLQLKPGMR VLDIGCGWGG LAHYMASNYD VSVVGVTISA EQQKMAQERC EGLDVTILLQ DYRDLNDQFD RIVSVGMFEH VGPKNYDTYF AVVDRNLKPE GIFLLHTIGS KKTDLNVDPW INKYIFPNGC LPSVRQIAQS SEPHFVMEDW HNFGADYDTT LMAWYERFLA AWPEIADNYS ERFKRMFTYY LNACAGAFRA RDIQLWQVVF SRGVENGLRV AR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge.
  • 基因功能參考文獻：
    1. both chemical steps of this enzymatic cyclopropanation, the methyl addition onto the double bond and the deprotonation step, are rate determining, a common situation in efficient enzymes. PMID: 20538038
    2. Experiments are described that distinguish between two mechanistic scenarios for the cyclopropanation of unactivated olefins by cyclopropane fatty acid synthase. PMID: 15491158
    3. report mutagenetic and chemical rescue experiments that confirm an important role for bicarbonate in E. coli CFAS catalysis; propose that residue E239, one of the bicarbonate ligands, could form a catalytic dyad with this ion PMID: 16216082
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    CFA/CMAS family
  • 數(shù)據(jù)庫鏈接：


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