在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli Alpha-ketoglutarate-dependent dioxygenase AlkB (alkB)

In Stock
  • 中文名稱:
    Recombinant Escherichia coli Alpha-ketoglutarate-dependent dioxygenase AlkB(alkB)
  • 貨號:
    CSB-EP356444ENV
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356444ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) alkB.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356444ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) alkB.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    alkB
  • Uniprot No.:
  • 別名:
    alkB; aidD; b2212; JW2200Alpha-ketoglutarate-dependent dioxygenase AlkB; EC 1.14.11.33; Alkylated DNA repair protein AlkB; DNA oxidative demethylase AlkB
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    44.1kDa
  • 表達區域:
    1-216aa
  • 氨基酸序列
    MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQAGKKE
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).
  • 基因功能參考文獻:
    1. SSB-AlkB interaction promotes faster repair of the methyl DNA adducts. PMID: 29326044
    2. AlkB has a wide variety of substrates, including monoalkyl and exocyclic bridged adducts. (Review) PMID: 26152727
    3. AlkB dioxygenase preferentially repairs protonated substrates: specificity against exocyclic adducts and molecular mechanism of action PMID: 23148216
    4. The AlkB dioxygenase plays a major role in decreasing the level of AT-->TA mutations, thus in the repair of Etheno-DNA adducts in E.coli cells. PMID: 21193516
    5. work provides direct evidence that AlkB suppresses both genotoxicity and mutagenesis by physiologically realistic low doses of 1-alkylpurine and 3-alkylpyrimidine DNA damage in vivo PMID: 15381779
    6. AlkB repairs epsilonA and epsilonC genomic lesions. PMID: 16200073
    7. crystal structures of substrate and product complexes of E. coli AlkB at resolutions from 1.8 to 2.3 A PMID: 16482161
    8. AlkB is shown here to be a geno-protective agent that reduces the toxicity of DNA damage by converting the primary adduct to a less toxic secondary product PMID: 17213319
    9. Cocrystal structures provide insight into the structural basis of this "k(cat)/K(m) compensation," which makes a significant contribution to promiscuous substrate recognition by AlkB. PMID: 19706517

    顯示更多

    收起更多

  • 蛋白家族:
    AlkB family
  • 數據庫鏈接:


主站蜘蛛池模板: 亚洲综合久久精品无码色欲| 精品人伦一区二区三电影| 影音先锋中文字幕人妻| 欧美疯狂性受xxxxx另类| 婷婷久久综合九色综合绿巨人| 老湿机国产福利视频| 无码专区丰满人妻斩六十路| 日韩人妻无码精品-专区| 女邻居丰满的奶水| 精品国产乱码久久久久久夜深人妻| 超caopor在线公开视频| 一区二区传媒有限公司| 久久婷婷五月综合色99啪| 老司机久久99久久精品播放| av无码人妻波多野结衣| 国产爆乳成av人在线播放| 麻豆av一区二区三区| 日韩人妻无码一区二区三区综合部 | 亚洲视频高清不卡在线观看| 98久9在线 | 免费| 少妇真实被内射视频三四区| 少妇挑战黑人高潮惨叫| 综合色区国产亚洲另类| 与子敌伦刺激对白播放| 欧洲熟妇色xxxx欧美老妇| 曰韩欧美群交p片内射| 中国国产1级毛卡片| 亚洲精品乱码久久久久久中文字幕| 亚洲视频在线观看| 无遮挡呻吟娇喘视频免费播放| 国产美熟女乱又伦av果冻传媒| 欧美性色黄大片| 日本高清www午色夜com| 色avav色av爱avav亚洲色拍| 欧美另类精品xxxx| 麻豆精产国品| 国内揄拍国内精品对白| 亚洲综合久久一本伊一区| 国产男女猛烈无遮挡免费网站| 国产亚洲视频在线播放香蕉| 人妻系列无码一区二区三区|