在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli Alpha-ketoglutarate-dependent dioxygenase AlkB (alkB)

In Stock
  • 中文名稱:
    Recombinant Escherichia coli Alpha-ketoglutarate-dependent dioxygenase AlkB(alkB)
  • 貨號:
    CSB-EP356444ENV
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356444ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) alkB.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356444ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) alkB.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    alkB
  • Uniprot No.:
  • 別名:
    alkB; aidD; b2212; JW2200Alpha-ketoglutarate-dependent dioxygenase AlkB; EC 1.14.11.33; Alkylated DNA repair protein AlkB; DNA oxidative demethylase AlkB
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    44.1kDa
  • 表達區域:
    1-216aa
  • 氨基酸序列
    MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQAGKKE
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).
  • 基因功能參考文獻:
    1. SSB-AlkB interaction promotes faster repair of the methyl DNA adducts. PMID: 29326044
    2. AlkB has a wide variety of substrates, including monoalkyl and exocyclic bridged adducts. (Review) PMID: 26152727
    3. AlkB dioxygenase preferentially repairs protonated substrates: specificity against exocyclic adducts and molecular mechanism of action PMID: 23148216
    4. The AlkB dioxygenase plays a major role in decreasing the level of AT-->TA mutations, thus in the repair of Etheno-DNA adducts in E.coli cells. PMID: 21193516
    5. work provides direct evidence that AlkB suppresses both genotoxicity and mutagenesis by physiologically realistic low doses of 1-alkylpurine and 3-alkylpyrimidine DNA damage in vivo PMID: 15381779
    6. AlkB repairs epsilonA and epsilonC genomic lesions. PMID: 16200073
    7. crystal structures of substrate and product complexes of E. coli AlkB at resolutions from 1.8 to 2.3 A PMID: 16482161
    8. AlkB is shown here to be a geno-protective agent that reduces the toxicity of DNA damage by converting the primary adduct to a less toxic secondary product PMID: 17213319
    9. Cocrystal structures provide insight into the structural basis of this "k(cat)/K(m) compensation," which makes a significant contribution to promiscuous substrate recognition by AlkB. PMID: 19706517

    顯示更多

    收起更多

  • 蛋白家族:
    AlkB family
  • 數據庫鏈接:


主站蜘蛛池模板: 69天堂人成无码免费视频| 亚洲国产成人久久一区久久| 久久天天躁夜夜躁狠狠综合| 免费大香伊蕉在人线国产卡| 日本熟妇厨房bbw| 日韩丰满少妇无码内射| 131美女爱做视频| 色综合久久久久综合99| 久久精品国产只有精品2020| 亚洲综合激情七月婷婷| 日韩毛片免费无码无毒视频观看| 欧美人与物videos另类xxxxx| 亚洲国产制服丝袜无码av| 国产在线看片免费视频| 九九视频麻婆豆腐在线观看| 久久久无码精品亚洲日韩按摩| 老熟女乱子伦| 苍井空亚洲精品aa片在线播放| 成人精品视频在线观看不卡| 日日大香人伊一本线久| 曰批视频免费30分钟成人| 亚洲色婷婷久久精品av蜜桃久久| 无码人妻精品中文字幕| 无码熟熟妇丰满人妻啪啪| 亚洲中文字幕乱码熟女在线| 台湾无码一区二区| 97人妻精品一区二区三区| 无码人妻丰满熟妇区毛片18 | 99久久久无码国产精品不卡| 亚洲日韩精品欧美一区二区一| 99re6热在线精品视频观看| 免费无码av片在线观看网址| 香蕉久久人人爽人人爽人人片av| 久久久综合亚洲色一区二区三区| 亚洲第一极品精品无码| 夜夜欢性恔真人免费视频| 久久天天躁狠狠躁夜夜2020一| 精品亚洲成a人片在线观看少妇| 亚洲国产欧美一区点击进入| 久久欧美一区二区三区性牲奴| 国产9 9在线 | 欧洲|