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Recombinant Escherichia coli Aerotaxis receptor (aer), partial

  • 中文名稱:
    Recombinant Escherichia coli Aerotaxis receptor(aer),partial
  • 貨號:
    CSB-YP342683ENV1
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli Aerotaxis receptor(aer),partial
  • 貨號:
    CSB-EP342683ENV1
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli Aerotaxis receptor(aer),partial
  • 貨號:
    CSB-EP342683ENV1-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli Aerotaxis receptor(aer),partial
  • 貨號:
    CSB-BP342683ENV1
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli Aerotaxis receptor(aer),partial
  • 貨號:
    CSB-MP342683ENV1
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    aer
  • Uniprot No.:
  • 別名:
    aer; air; yqjJ; b3072; JW3043; Aerotaxis receptor
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Signal transducer for aerotaxis. The aerotactic response is the accumulation of cells around air bubbles. The nature of the sensory stimulus detected by this protein is the proton motive force or cellular redox state. It uses a FAD prosthetic group as a redox sensor to monitor oxygen levels.
  • 基因功能參考文獻:
    1. Oxidized Aer activates CheA, whereas ASQ Aer reversibly inhibits CheA. PMID: 27803157
    2. These studies demonstrated trigonal interactions among the F1 linkers from three Aer monomers, presumably within trimer-of-dimer units, as well as binary interactions between subunits. PMID: 21097634
    3. Gain-of-function mutations are associated with the signalling pathway in the PAS domain of Aer. PMID: 20545849
    4. functional interactions between the PAS domain and the HAMP AS-2 helix are required for FAD binding and aerotactic signaling by Aer. PMID: 15489456
    5. Dimers also formed in mutants that did not bind flavin adenine dinucleotide and in truncated peptides without a signaling domain and part of the HAMP domain. PMID: 15489458
    6. Either the PAS or signalling domains could be deleted from the non-signalling subunit of the Aer heterodimer, but removing 16 residues from the C-terminus of the signalling subunit abolished aerotaxis. PMID: 16430703
    7. Mutations and truncations in the sequence encoding residues 15 to 21 introduced a range of phenotypes, including defects in FAD binding, constant tumbling motility, and an inverse response in which E. coli cells migrated away from oxygen concentrations PMID: 16513745
    8. EilA activates the genetically linked high molecular weight bacterial surface protein Air PMID: 16762026
    9. strong Aer responses to oxygen are associated with redox changes in NADH dehydrogenase I PMID: 16995896
    10. the overall membrane organization of Aer included a maximum of three Aer dimers, did not swap neighbors over time, and appeared to be constrained by interactions in the cytosolic signaling domain. PMID: 17693513
    11. Data confirmed that the Aer HAMP domain is composed of two alpha-helices separated by a structured loop and significance of the HAMP and proximal signaling domain structure for signal transduction is discussed. PMID: 18203838
    12. The aer mutant exhibited a decreased ability to colonize the intestine when compared to wild-type cells. PMID: 19130287

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  • 亞細胞定位:
    Cell inner membrane; Multi-pass membrane protein. Note=Predominantly localized to one cell pole in mid-to-late exponential phase, with a few smaller foci elsewhere in the cell.
  • 數據庫鏈接:


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