Recombinant Escherichia coli Adenylosuccinate synthetase (purA)

中文名稱：

大腸桿菌purA重組蛋白
貨號：

CSB-YP363991ENV
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌purA重組蛋白
貨號：

CSB-EP363991ENV
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌purA重組蛋白
貨號：

CSB-EP363991ENV-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌purA重組蛋白
貨號：

CSB-BP363991ENV
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌purA重組蛋白
貨號：

CSB-MP363991ENV
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

purA
Uniprot No.：

P0A7D4
別名：

purA; adeK; b4177; JW4135; Adenylosuccinate synthetase; AMPSase; AdSS; EC 6.3.4.4; IMP--aspartate ligase
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-432
氨基酸序列

GNNVVVLGT QWGDEGKGKI VDLLTERAKY VVRYQGGHNA GHTLVINGEK TVLHLIPSGI LRENVTSIIG NGVVLSPAAL MKEMKELEDR GIPVRERLLL SEACPLILDY HVALDNAREK ARGAKAIGTT GRGIGPAYED KVARRGLRVG DLFDKETFAE KLKEVMEYHN FQLVNYYKAE AVDYQKVLDD TMAVADILTS MVVDVSDLLD QARQRGDFVM FEGAQGTLLD IDHGTYPYVT SSNTTAGGVA TGSGLGPRYV DYVLGILKAY STRVGAGPFP TELFDETGEF LCKQGNEFGA TTGRRRRTGW LDTVAVRRAV QLNSLSGFCL TKLDVLDGLK EVKLCVAYRM PDGREVTTTP LAADDWKGVE PIYETMPGWS ESTFGVKDRS GLPQAALNYI KRIEELTGVP IDIISTGPDR TETMILRDPF DA
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.
基因功能參考文獻：
1. The absence of the 2'-hydroxyl group of the deoxyribonucleotide destabilizes binding of the ligand to the L-aspartate pocket of adenylosuccinate synthetase by disrupting hydrogen bonds and by introducing a cavity into the fully ligated active site. PMID: 16981730
亞細胞定位：

Cytoplasm.
蛋白家族：

Adenylosuccinate synthetase family
數據庫鏈接：

KEGG: ecj:JW4135

STRING: 316385.ECDH10B_4372