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Recombinant Escherichia coli ATP synthase subunit beta (atpD)

  • 中文名稱:
    Recombinant Escherichia coli ATP synthase subunit beta(atpD)
  • 貨號:
    CSB-YP002350ENV
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli ATP synthase subunit beta(atpD)
  • 貨號:
    CSB-EP002350ENV
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli ATP synthase subunit beta(atpD)
  • 貨號:
    CSB-EP002350ENV-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli ATP synthase subunit beta(atpD)
  • 貨號:
    CSB-BP002350ENV
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli ATP synthase subunit beta(atpD)
  • 貨號:
    CSB-MP002350ENV
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    atpD
  • Uniprot No.:
  • 別名:
    atpD; papB; uncD; b3732; JW3710ATP synthase subunit beta; EC 7.1.2.2; ATP synthase F1 sector subunit beta; F-ATPase subunit beta
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    Full Length of Mature Protein
  • 表達區域:
    2-460
  • 氨基酸序列
    ATGKIVQVI GAVVDVEFPQ DAVPRVYDAL EVQNGNERLV LEVQQQLGGG IVRTIAMGSS DGLRRGLDVK DLEHPIEVPV GKATLGRIMN VLGEPVDMKG EIGEEERWAI HRAAPSYEEL SNSQELLETG IKVIDLMCPF AKGGKVGLFG GAGVGKTVNM MELIRNIAIE HSGYSVFAGV GERTREGNDF YHEMTDSNVI DKVSLVYGQM NEPPGNRLRV ALTGLTMAEK FRDEGRDVLL FVDNIYRYTL AGTEVSALLG RMPSAVGYQP TLAEEMGVLQ ERITSTKTGS ITSVQAVYVP ADDLTDPSPA TTFAHLDATV VLSRQIASLG IYPAVDPLDS TSRQLDPLVV GQEHYDTARG VQSILQRYQE LKDIIAILGM DELSEEDKLV VARARKIQRF LSQPFFVAEV FTGSPGKYVS LKDTIRGFKG IMEGEYDHLP EQAFYMVGSI EEAVEKAKKL
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.
  • 基因功能參考文獻:
    1. The results show that beta/gamma rotor-stator interactions near the gamma carboxyl terminus and at the gammaMet23-betaDELSEED both contribute to formation of the rate-limiting transition state of rotational catalysis. PMID: 22582396
    2. the interaction of F(0)F(1) ATP synthase with FliG is important for the function of the switch of bacterial flagella PMID: 22210351
    3. Homology modeling indicates that the amino acid replacement induces a hydrophobic network, in which the betaMet159, betaIle163, and betaAla167 residues of the beta subunit are involved together with the mutant betaPhe174. PMID: 20331967
    4. These results imply that the polypeptide in the b N-terminus position is more important for F1 binding than the one in the b C-terminus position and illustrate the significance of the asymmetry of the b dimer in the enzyme. PMID: 17766239
    5. The authors identified a knockout of atpD, coding for a component of the F(o)F(1) ATPase, as defective in Ca(2+) efflux. PMID: 19481094

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  • 亞細胞定位:
    Cell inner membrane; Peripheral membrane protein.
  • 蛋白家族:
    ATPase alpha/beta chains family
  • 數據庫鏈接:


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