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  4. Recombinant Escherichia coli ATP-dependent RNA helicase rhlE (rhlE)

Recombinant Escherichia coli ATP-dependent RNA helicase rhlE (rhlE)

  • 中文名稱：
    大腸桿菌rhlE重組蛋白
  • 貨號：
    CSB-YP335156ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌rhlE重組蛋白
  • 貨號：
    CSB-EP335156ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌rhlE重組蛋白
  • 貨號：
    CSB-EP335156ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌rhlE重組蛋白
  • 貨號：
    CSB-BP335156ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌rhlE重組蛋白
  • 貨號：
    CSB-MP335156ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rhlE
  • Uniprot No.：
  • 別名：
    rhlE; b0797; JW0781; ATP-dependent RNA helicase RhlE; EC 3.6.4.13
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-454
  • 氨基酸序列
    MSFDSLGLSP DILRAVAEQG YREPTPIQQQ AIPAVLEGRD LMASAQTGTG KTAGFTLPLL QHLITRQPHA KGRRPVRALI LTPTRELAAQ IGENVRDYSK YLNIRSLVVF GGVSINPQMM KLRGGVDVLV ATPGRLLDLE HQNAVKLDQV EILVLDEADR MLDMGFIHDI RRVLTKLPAK RQNLLFSATF SDDIKALAEK LLHNPLEIEV ARRNTASDQV TQHVHFVDKK RKRELLSHMI GKGNWQQVLV FTRTKHGANH LAEQLNKDGI RSAAIHGNKS QGARTRALAD FKSGDIRVLV ATDIAARGLD IEELPHVVNY ELPNVPEDYV HRIGRTGRAA ATGEALSLVC VDEHKLLRDI EKLLKKEIPR IAIPGYEPDP SIKAEPIQNG RQQRGGGGRG QGGGRGQQQP RRGEGGAKSA SAKPAEKPSR RLGDAKPAGE QQRRRRPRKP AAAQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DEAD-box RNA helicase involved in ribosome assembly. Has RNA-dependent ATPase activity and unwinds double-stranded RNA. May play a role in the interconversion of ribosomal RNA-folding intermediates that are further processed by DeaD or SrmB during ribosome maturation.
  • 基因功能參考文獻：
    1. presented in vitro evidence that SrmB, RhlE and CsdA can bind Rnase E at a site that is different from the rhlB binding site; RhlE can replace the function of RhlB in vitro PMID: 15554979
    2. RhlE is a novel ribosome assembly factor, which plays a role in the interconversion of ribosomal RNA-folding intermediates that are further processed by DeaD or SrmB during ribosome maturation. PMID: 18083833
  • 亞細胞定位：
    Cytoplasm. Note=Ribosome-associated. Can either bind to the intact 70S ribosome or to individual ribosomal subunits.
  • 蛋白家族：
    DEAD box helicase family, RhlE subfamily
  • 數據庫鏈接：


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