Recombinant Escherichia coli 4-hydroxy-tetrahydrodipicolinate synthase (dapA)

中文名稱：

Recombinant Escherichia coli 4-hydroxy-tetrahydrodipicolinate synthase(dapA)
貨號：

CSB-RP185174Ba
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

dapA
Uniprot No.：

P0A6L2
別名：

dapA; b2478; JW2463; 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length
來源：

E.coli
分子量：

35.3kDa
表達區域：

1-292aa
氨基酸序列

MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGTTGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLTVTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGIKEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMCKLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITDSGRETVRAALKHAGLL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).
基因功能參考文獻：
1. The dapA promoter is activated by diaminopimelic acid. PMID: 15158272
2. crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from E. coli are presented to 1.9 and 2.0 A, respectively PMID: 16041077
3. evidence that Arg138 of dihydrodipicolinate synthase is responsible for binding the carboxyl of (S)-ASA and is not involved in the mechanism of (S)-lysine inhibition PMID: 16185069
4. Results suggest that the homotetrameric structure of dihydrodipicolinate synthase reduces dynamic fluctuations present in the dimeric forms and increases specificity for the first substrate, pyruvate. PMID: 18556019
5. to probe the mechanism of dihydrodipicolinate synthase (DHDPS), inhibition of DHDPS by the substrate analog, beta-hydroxypyruvate was studied; crystal structure of DHDPS complexed with beta-hydroxypyruvate was solved PMID: 18787203
6. This study investigated the C-terminal domain of E. coli DHDPS by characterising a C-terminal truncated DHDPS (DHDPS-H225*). PMID: 19338756
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亞細胞定位：

Cytoplasm.
蛋白家族：

DapA family
數據庫鏈接：

KEGG: ecj:JW2463

STRING: 316385.ECDH10B_2644