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Recombinant Enterobacteria phage lambda Repressor protein CI (CI)

  • 中文名稱:
    Recombinant Enterobacteria phage lambda Repressor protein CI(CI)
  • 貨號:
    CSB-YP355929ECW
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Enterobacteria phage lambda Repressor protein CI(CI)
  • 貨號:
    CSB-EP355929ECW
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Enterobacteria phage lambda Repressor protein CI(CI)
  • 貨號:
    CSB-EP355929ECW-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Enterobacteria phage lambda Repressor protein CI(CI)
  • 貨號:
    CSB-BP355929ECW
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Enterobacteria phage lambda Repressor protein CI(CI)
  • 貨號:
    CSB-MP355929ECW
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    cI
  • Uniprot No.:
  • 別名:
    cI; lambdap88; Repressor protein cI
  • 種屬:
    Enterobacteria phage lambda (Bacteriophage lambda)
  • 蛋白長度:
    Full Length of Mature Protein
  • 表達區(qū)域:
    2-237
  • 氨基酸序列
    STKKKPLTQ EQLEDARRLK AIYEKKKNEL GLSQESVADK MGMGQSGVGA LFNGINALNA YNAALLAKIL KVSVEEFSPS IAREIYEMYE AVSMQPSLRS EYEYPVFSHV QAGMFSPELR TFTKGDAERW VSTTKKASDS AFWLEVEGNS MTAPTGSKPS FPDGMLILVD PEQAVEPGDF CIARLGGDEF TFKKLIRDSG QVFLQPLNPQ YPMIPCNESC SVVGKVIASQ WPEETFG
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Acts as a transcriptional repressor that allows virus to establish and maintain latency. Prevents both the viral DNA replication and the exit programs. Clamps the two operator OL (operator left made of OL1, OL2 and OL3 sites) and OR (operator right made of OR1, OR2 and OR3 sites) together by binding to them and arranging the intervening DNA in a loop. This step allows repression of lytic pR and pL promoters by binding to OL1, OL2, OR1 and OR2 simultaneously. The binding of cI on OR2 additionally activates the transcription of the cI gene thereby mediating an autoregulatory function to maintain the latent state. Once cI is present in sufficient amount, it can repress its own transcription by binding to OL3 and OR3.
  • 基因功能參考文獻:
    1. Enterobacteria phage lambda repressor fragment (6-85) takes only microseconds to reach its native state from an extensively unfolded state, while the latter takes milliseconds to reach compact beta-rich traps. PMID: 22066714
    2. Temperature-dependent interactions (e.g., hydrophobicity) play a large role in the initial stages of folding of lambda repressor N-terminal domain (residues 6-85) but not in the initial stages of unfolding. PMID: 15476395
    3. Two-state folding of lambda(6-85) is not a robust process. PMID: 17075136
    4. NMR was used to characterize the secondary structure and dynamics of MetO-lambdaLS in standard phosphate buffer PMID: 17260944
  • 數(shù)據庫鏈接:

    KEGG: vg:3827059



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