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  4. Recombinant Enterobacteria phage T7 DNA polymerase (5), partial

Recombinant Enterobacteria phage T7 DNA polymerase (5), partial

  • 中文名稱：
    Recombinant Enterobacteria phage T7 DNA polymerase(5) ,partial
  • 貨號：
    CSB-YP365450EEB
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage T7 DNA polymerase(5) ,partial
  • 貨號：
    CSB-EP365450EEB
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage T7 DNA polymerase(5) ,partial
  • 貨號：
    CSB-EP365450EEB-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage T7 DNA polymerase(5) ,partial
  • 貨號：
    CSB-BP365450EEB
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage T7 DNA polymerase(5) ,partial
  • 貨號：
    CSB-MP365450EEB
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    5
  • Uniprot No.：
  • 別名：
    5DNA-directed DNA polymerase; EC 2.7.7.7; EC 3.1.11.-; Gene product 5; Gp5
  • 種屬：
    Enterobacteria phage T7 (Bacteriophage T7)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Replicates viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'-5' direction. Non-processive DNA polymerase that achieves processivity by binding to host thioredoxin (TrxA). This interaction increases the rate of dNTP incorporation to yield a processivity of approximately 800 nucleotides (nt) per binding event. Interacts with DNA helicase gp4 to coordinate nucleotide polymerization with unwinding of the DNA. The leading strand is synthesized continuously while synthesis of the lagging strand requires the synthesis of oligoribonucleotides by the primase domain of gp4.
  • 基因功能參考文獻：
    1. Data suggest replication loop is released at nick formed in lagging-strand DNA when polymerase (gp5) encounters previously synthesized Okazaki fragment (OF), releasing lagging-strand DNA, and retaining gp5 at replication fork for synthesis of next OF. PMID: 26620561
    2. Results suggest that the interaction between the C-terminal tail of the helicase and the basic patch of gp5 is critical for initiation of strand-displacement synthesis. PMID: 21606333
    3. Small angle X-ray scattering studies combined with nuclease footprinting reveal two conformations of T7 DNA polymerase, one in the free state and one upon binding to E. coli thioredoxin PMID: 20696935
    4. single-molecule imaging approach to visualize the interaction of fluorescently labeled T7 DNA polymerase with double-stranded DNA PMID: 20080681
    5. the thioredoxin-binding domain is a molecular switch that regulates the interaction of T7 DNA polymerase with other proteins of the replisome PMID: 15795374
    6. Escherichia coli thioredoxin, the processivity factor, binds with bacteriophage T7 DNA polymerase and helicase PMID: 18757858
    7. Results describe the nucleotidyl transfer reaction mechanism in the well-characterized high-fidelity replicative DNA polymerase from phage T7. PMID: 19389406

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  • 蛋白家族：
    DNA polymerase type-A family
  • 數據庫鏈接：

    KEGG: vg:1261044



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