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  4. Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda)

Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda)

  • 中文名稱：
    Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda(dda)
  • 貨號：
    CSB-YP329153EDZ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda(dda)
  • 貨號：
    CSB-EP329153EDZ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda(dda)
  • 貨號：
    CSB-BP329153EDZ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda(dda)
  • 貨號：
    CSB-MP329153EDZ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dda
  • Uniprot No.：
  • 別名：
    dda; sudATP-dependent DNA helicase dda; EC 3.6.4.12
  • 種屬：
    Enterobacteria phage T4 (Bacteriophage T4)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-439
  • 氨基酸序列
    MTFDDLTEGQ KNAFNIVMKA IKEKKHHVTI NGPAGTGKTT LTKFIIEALI STGGTGIILA APTHAAKKIL SKLSGKEAST IHSILKINPV TYEENVLFEQ KEVPDLAKCR VLICDEVSMY DRKLFKILLS TIPPWCTIIG IGDNKQIRPV EPGENTAYIS PFFTHKDFYQ CELTEVKRSN APIIDVATDV RNGKWNYDKV VDGHGVRGFT GDTALRDFMV NYFSIVKSLD DLFENRVMAF TNKSVDKLNS IIRKKIFETD KDFIVGEIIV MQEPLFKTYK IDGKPVSEII FNNGQLVRII EAEYTSTFVK ARGVPGEYLI RHWDLTVETY GDDEYYREKI KIISSDEELY KFNLFLAKTA ETYKNWNKGG KAPWSDFWDA KSQFSKVKAL PASTFHKAQG MSVDRAFIYT PCIHYADVEL AQQLLYVGVT RGRYDVFYV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA.
  • 基因功能參考文獻：
    1. Gp32 plays both positive and negative roles in regulating Dda helicase activity, and that both effects may influence the outcome of complex DNA transactions such as replication fork restart. PMID: 25481875
    2. Dda cross-links to DNA in the 167-199 Peptide. PMID: 19256528
    3. Dda suffice to rescue stalled T4 replication forks in vitro PMID: 15194689
    4. Enhanced binding to the longer single-stranded overhangs was not responsible for enhanced processivity under conditions of excess Dda enzyme. Rather, multiple molecules of Dda bound to the same substrate exhibit greater processivity for DNA unwinding. PMID: 16185067
    5. observations demonstrate a complex mechanism containing nonuniform steps for a monomeric helicase PMID: 16474403
    6. Multiple Dda molecules act to displace DNA binding proteins in a manner that correlates with the DNA unwinding activity and streptavidin displacement activity. PMID: 16738140

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  • 數據庫鏈接：

    KEGG: vg:1258784



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