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  4. Recombinant Escherichia phage MS2 Capsid protein

Recombinant Escherichia phage MS2 Capsid protein

  • 中文名稱：
    Recombinant Escherichia phage MS2 Capsid protein
  • 貨號：
    CSB-YP365998ECZ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Escherichia phage MS2 Capsid protein
  • 貨號：
    CSB-EP365998ECZ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Escherichia phage MS2 Capsid protein
  • 貨號：
    CSB-BP365998ECZ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Escherichia phage MS2 Capsid protein
  • 貨號：
    CSB-MP365998ECZ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    Capsid protein; CP; Coat protein
  • 種屬：
    Enterobacteria phage MS2 (Bacteriophage MS2)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-130
  • 氨基酸序列
    ASNFTQFVL VDNGGTGDVT VAPSNFANGV AEWISSNSRS QAYKVTCSVR QSSAQNRKYT IKVEVPKVAT QTVGGVELPV AAWRSYLNME LTIPIFATNS DCELIVKAMQ GLLKDGNPIP SAIAANSGIY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

I would like to know if “Preferably a protein fused to maltose binding protein or a GST tagged protein are better.” Can this be done?

A:
Thank you for your inquiry. We can meet your demand to provide a protein fused to maltose binding protein or a GST tagged protein, pls remark this requirement when placing the order.
There isn't any extra charges, and the lead time won't be extended.

靶點詳情

  • 功能：
    Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 26 nm in diameter, and consisting of 89 capsid proteins dimers (178 capsid proteins). Involved in viral genome encapsidation through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome. The capsid contains also 1 copy of the A2 maturation protein.; Acts as a translational repressor of viral replicase synthesis late in infection. This latter function is the result of capsid protein interaction with an RNA hairpin which contains the replicase ribosome-binding site.
  • 基因功能參考文獻：
    1. When the MS2 capsid assembles, the conformation of bound RNA and its interaction with the PRR1 coat-protein dimer are similar in both small RNA phages PRR1 and phage MS2. PMID: 23519411
    2. A new concept in virus biology provides predictive information on structural constraints on coat protein and genome topography, revealing a previously unrecognized structural interdependence of the shapes and sizes of different viral components. PMID: 23403965
    3. This study shows that translational repressor binding results in self-association of asymmetric (AB) dimers being inhibited, whilst association of AB with symmetric (CC) dimers is greatly enhanced. PMID: 20562027
    4. MS2 coat protein binds several Escherichia coli mRNAs in vitro PMID: 11058143
  • 亞細胞定位：
    Virion.
  • 蛋白家族：
    Levivirus capsid protein family
  • 數據庫鏈接：

    KEGG: vg:1260899



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