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  4. Recombinant Emericella nidulans tRNA (guanine (37)-N1)-methyltransferase

Recombinant Emericella nidulans tRNA (guanine (37)-N1)-methyltransferase

  • 中文名稱：
    構巢裸孢殼trm5重組蛋白
  • 貨號：
    CSB-YP513897EKF
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    構巢裸孢殼trm5重組蛋白
  • 貨號：
    CSB-EP513897EKF
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    構巢裸孢殼trm5重組蛋白
  • 貨號：
    CSB-EP513897EKF-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    構巢裸孢殼trm5重組蛋白
  • 貨號：
    CSB-BP513897EKF
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    構巢裸孢殼trm5重組蛋白
  • 貨號：
    CSB-MP513897EKF
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    trm5
  • Uniprot No.：
  • 別名：
    trm5; AN10361; tRNA; guanine(37)-N1)-methyltransferase; EC 2.1.1.228; M1G-methyltransferase; tRNA [GM37] methyltransferase; tRNA methyltransferase 5
  • 種屬：
    Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-478
  • 氨基酸序列
    MAQSTEDNTR RPRPSDLPEM FRPPVNRAMR VLDRSFFKKT VPLSAATIFK NSDISRVRRA LEQSKDILAL PRLNTIQDIK QDDVVKKCLL LKETIRHDDT ATWSPTINEL VDNGVVGVGP FDLTLDYDYW LHSDIISAVL PEELLEEVPQ GFTQVGHVAQ LNLREQFIPW RHLIAQVLLD KNPTLRTVIR KTEDVGSQSE FRTFPYELLA GDSDMNVIQH EQDCEFRFDF SRVYWNSRLH TEHQRLVDLF KPGEMVCDVM AGVGPFAIPA GKKKIFVWAN DLNPHGYEVM QDAVKRNKVF KFVTPFNQDG RSFIRWSARA LQKYDPVTVT IQPRTKRTRD ASGQVKETQP PLEVYTRPKV FHHYVMNLPG NALEFLDAFI GVYAGCEELF EPHTKEQLPM VHVYCFSGHS ENEVDDHIDI CKRMSERLEY PITVEDRVGG AGNTELELSI HNVRLVSPNK QMFCASFRLP RAVAFRKK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
  • 亞細胞定位：
    Mitochondrion matrix. Nucleus. Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, TRM5/TYW2 family
  • 數據庫鏈接：


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