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  4. Recombinant E3 ubiquitin-protein ligase ipaH9.8 (ipaH9.8), partial

Recombinant E3 ubiquitin-protein ligase ipaH9.8 (ipaH9.8), partial

  • 中文名稱：
    弗累克斯訥氏桿菌ipaH9.8重組蛋白
  • 貨號：
    CSB-YP855379SZB
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    弗累克斯訥氏桿菌ipaH9.8重組蛋白
  • 貨號：
    CSB-EP855379SZB
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    弗累克斯訥氏桿菌ipaH9.8重組蛋白
  • 貨號：
    CSB-EP855379SZB-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    弗累克斯訥氏桿菌ipaH9.8重組蛋白
  • 貨號：
    CSB-BP855379SZB
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    弗累克斯訥氏桿菌ipaH9.8重組蛋白
  • 貨號：
    CSB-MP855379SZB
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ipaH9.8
  • Uniprot No.：
  • 別名：
    ipaH9.8; CP0226; pWR501_0234; SFLP090; E3 ubiquitin-protein ligase ipaH9.8; EC 2.3.2.27; Invasion plasmid antigen ipaH9.8; RING-type E3 ubiquitin transferase ipaH9.8
  • 種屬：
    Shigella flexneri
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway and modulates the acute inflammatory responses, thus facilitating bacterial colonization within the host cell. Interacts with IKBKG (NEMO) and TNIP1 (ABIN-1), a ubiquitin-binding adapter protein, which results in TNIP1-dependent 'Lys-27'-linked polyubiquitination of IKBKG. Consequently, polyubiquitinated IKBKG undergoes proteasome-dependent degradation, which perturbs NF-kappa-B activation during bacterial infection. Uses UBE2D2 (UBCH5B) as an E2 ubiquitin-conjugating enzyme. Mediates polyubiquitination of host U2AF1, leading to its proteasomal degradation.
  • 基因功能參考文獻：
    1. under non-reducing conditions IpaH9.8 undergoes a domain swap driven by the formation of a disulfide bridge involving the catalytic cysteine and that this dimer is unable to catalyse the ubiquitination of U2AF35 PMID: 20831869
  • 亞細胞定位：
    Secreted. Host cytoplasm. Host nucleus. Note=Secreted via Mxi-Spa type III secretion system (TTSS), and delivered into the host cytoplasm. Transported into the host nucleus. This transport is independent of cytosolic factors, but dependent on temperature and partly on ATP/GTP.
  • 蛋白家族：
    LRR-containing bacterial E3 ligase family
  • 數據庫鏈接：

    KEGG: sfl:CP0226



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