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  4. Recombinant Drosophila melanogaster WAS protein family homolog 1 (wash)

Recombinant Drosophila melanogaster WAS protein family homolog 1 (wash)

  • 中文名稱：
    黑腹果蠅wash重組蛋白
  • 貨號：
    CSB-YP025972DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅wash重組蛋白
  • 貨號：
    CSB-EP025972DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅wash重組蛋白
  • 貨號：
    CSB-EP025972DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅wash重組蛋白
  • 貨號：
    CSB-BP025972DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅wash重組蛋白
  • 貨號：
    CSB-MP025972DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    wash
  • Uniprot No.：
  • 別名：
    wash; CG13176WASH complex subunit 1; Protein washout; WAS protein family homolog 1
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-499
  • 氨基酸序列
    MEESPYLHSP YQVAIIATDL HHEDTIIQAA QSLDCLHKTI NSIFERIDAR LARNGSKVED INNRVKRAQA KIDALVGSKR AIQIFAPARF PASDVLAPLP ATFPQVAANP LMEQQVDQLP QGTYSSHSAA DQKPDDADIF FHVRGDREQE SPLVAERKIT NRTAGLGILP AGGVRSVPSL MRFNTNEFAY GEDLNAWKRS LPPQNARRVA SQSTQLTGEK QLAPAPHSLA HGTTKLATPA GDLRYNPAAL AAPAIDVPLD LPDLPGIAND LQYEPVEEQT PIAPSQQFGD LPELPDLGLE EQDIIVQAIA AQTHIPGPVR RKSVGQCPSP VTAAPPPPPP PPPPPPPPPP AQTSAIPSPP PFPTKGAVKP LSPSLATPLN MPQPPPATED PRSELMAAIR NAGGVHGGRL RSPAAAPLDV VDNSRSKAGG AVTGDLMADL HNKLMLRRKG ISGSQNPVEA TAGNPLMQQL SRVIPPPVQP RKGSKSSDEH SEDDEDGWN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as a nucleation-promoting factor by activating the Arp2/3 complex to induce actin polymerization. Participates in both linear- and branched-actin networks. Functions in linear-filament (bundled F-actin) by acting downstream of Rho1 and regulating actin and microtubule organization during oogenesis. Nucleates actin in an Arp2/3-dependent manner and exhibits F-actin and microtubule bundling and cross-linking activity in the egg chamber. During embryogenesis, acts downstream of Rho1 to activate the Arp2/3 complex which is necessary for the developmental migration of tail hemocytes anteriorly along the ventral midline. Its function in hemocyte transmigration is independent of the WASH complex. May play a role in endosomal sorting; its assembly in the WASH complex may regulate its nucleation-promoting factor (NPF) activity.
  • 基因功能參考文獻：
    1. Drosophila WASH has conserved functions in integrin receptor recycling and lysosome neutralization. PMID: 27884932
    2. reveal a novel role for Wash in modulating nucleus morphology and in the organization of both chromatin and non-chromatin nuclear sub-structures PMID: 25754639
    3. Developmental hemocyte migration requires the interaction of Rho1 with its downstream effector Wash, a Wiskott-Aldrich syndrome family protein. PMID: 25739458
    4. Results establish Wash and Rho as regulators of both linear- and branched-actin networks, and suggest an Arp2/3-mediated mechanism for how cells might coordinately regulate these structures. PMID: 19633175
  • 蛋白家族：
    WASH1 family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG13176

    STRING: 7227.FBpp0087097

    UniGene: Dm.37984



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