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  4. Recombinant Drosophila melanogaster Voltage-dependent anion-selective channel (porin)

Recombinant Drosophila melanogaster Voltage-dependent anion-selective channel (porin)

  • 中文名稱：
    黑腹果蠅porin重組蛋白
  • 貨號：
    CSB-YP856714DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅porin重組蛋白
  • 貨號：
    CSB-EP856714DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅porin重組蛋白
  • 貨號：
    CSB-EP856714DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅porin重組蛋白
  • 貨號：
    CSB-BP856714DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅porin重組蛋白
  • 貨號：
    CSB-MP856714DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    porin
  • Uniprot No.：
  • 別名：
    porin; POR-1; VDAC; CG6647Voltage-dependent anion-selective channel; DmVDAC; Porin
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-282
  • 氨基酸序列
    APPSYSDLG KQARDIFSKG YNFGLWKLDL KTKTSSGIEF NTAGHSNQES GKVFGSLETK YKVKDYGLTL TEKWNTDNTL FTEVAVQDQL LEGLKLSLEG NFAPQSGNKN GKFKVAYGHE NVKADSDVNI DLKGPLINAS AVLGYQGWLA GYQTAFDTQQ SKLTTNNFAL GYTTKDFVLH TAVNDGQEFS GSIFQRTSDK LDVGVQLSWA SGTSNTKFAI GAKYQLDDDA SVRAKVNNAS QVGLGYQQKL RDGVTLTLST LVDGKNFNAG GHKIGVGLEL EA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 668
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. In depolarized mitochondria, acts downstream of park to promote mitophagy or prevent apoptosis; polyubiquitination by park promotes mitophagy, while monoubiquitination by park decreases mitochondrial calcium influx which ultimately inhibits apoptosis.
  • 基因功能參考文獻：
    1. The inhibition of porin phenocopies Parkinson disease phenotypes in Drosophila, while the overexpression of Buffy can counteract these phenotypes to improve the overall "healthspan" of the organism. PMID: 27881168
    2. Drosophila porin mutants have a role in mitochondrial dysfunction and disease PMID: 20110367
    3. identification of two PlacW insertions in the porin gene among a set of P-element insertions that have been mapped to the 32B3-4 region on the second chromosome PMID: 12207222
    4. Our data suggest that the PORIN protein is important in maintaining a photoreceptor response during prolonged stimulation. PMID: 17525991
    5. Porin isoform 1 is selectively targeted to follicular cells while Porin isoform 2 is present in mitochondria of the epithelial sheath cells of the ovariole. PMID: 18686020

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  • 亞細胞定位：
    Mitochondrion outer membrane.
  • 蛋白家族：
    Eukaryotic mitochondrial porin family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG6647

    STRING: 7227.FBpp0079772

    UniGene: Dm.33362



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