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Recombinant Drosophila melanogaster Ubiquitin carboxyl-terminal hydrolase 64E (Ubp64E), partial

  • 中文名稱:
    黑腹果蠅Usp47重組蛋白
  • 貨號:
    CSB-YP636559DLU
  • 說明書:
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅Usp47重組蛋白
  • 貨號:
    CSB-EP636559DLU
  • 說明書:
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅Usp47重組蛋白
  • 貨號:
    CSB-EP636559DLU-B
  • 說明書:
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅Usp47重組蛋白
  • 貨號:
    CSB-BP636559DLU
  • 說明書:
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅Usp47重組蛋白
  • 貨號:
    CSB-MP636559DLU
  • 說明書:
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    Usp47
  • Uniprot No.:
  • 別名:
    Usp47; Ubp64E; CG5486Ubiquitin carboxyl-terminal hydrolase 47; EC 3.4.19.12; Ubiquitin thioesterase 47; Ubiquitin-specific-processing protease 47
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Ubiquitin-specific protease that deubiquitinates target proteins to regulate different cellular and developmental pathways. Functions downstream of Dsor1/MEK to positively regulate the Ras/MAPK signaling pathway. Likely to modulate the pathway during various cellular and developmental processes including rl/MAPK activation by the receptors InR, Egfr and sevenless/sev. Functions in the post-translational stabilization of rl/MAPK levels in a mechanism that is independent of rl activity and opposes the activity of the E2 enzyme Unc6 and the putative E3 ligases poe, Ufd4 and Kcmf1, which mediate the ubiquitination and proteasomal degradation of rl. During eye development it may also act downstream of rl/MAPK to negatively regulate the Ras/MAPK signaling pathway by stabilizing the transcriptional repressor ttk and consequently inhibiting photoreceptor cell development. This suggests that at least during eye development, it may act in both the positive and negative regulation of the Ras/MAPK signaling pathway to mediate the development of different cell types. Positively regulates border follicle cell migration during oogenesis by mediating the deubiquitination and stabilization of slbo. In the wing disks it positively regulates wg signaling by stabilizing arm. Has an effect on position-effect variegation.
  • 基因功能參考文獻:
    1. In the presence of USP47, MAPK would be stabilized as USP47 acts to deubiquitinate MAPK, thus counteracting the direct ubiquitination of MAPK by POE/UBR4 activity. PMID: 27552662
  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    Peptidase C19 family
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG5486

    STRING: 7227.FBpp0076802

    UniGene: Dm.3704



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