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Recombinant Drosophila melanogaster Troponin T, skeletal muscle (up)

  • 中文名稱:
    黑腹果蠅up重組蛋白
  • 貨號:
    CSB-YP325666DLU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅up重組蛋白
  • 貨號:
    CSB-EP325666DLU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅up重組蛋白
  • 貨號:
    CSB-EP325666DLU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅up重組蛋白
  • 貨號:
    CSB-BP325666DLU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅up重組蛋白
  • 貨號:
    CSB-MP325666DLU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    up
  • Uniprot No.:
  • 別名:
    up; int; CG7107; Troponin T; skeletal muscle; Protein intended thorax; Protein upheld
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-397
  • 氨基酸序列
    MSDDEEYTSS EEEEVVEETR EETKPPQTPA EGEGDPEFIK RQDQKRSDLD DQLKEYITEW RKQRSKEEDE LKKLKEKQAK RKVTRAEEEQ KMAQRKKEEE ERRVREAEEK KQREIEEKRM RLEEAEKKRQ AMLQAMKDKD KKGPNFTIAK KDAGVLGLSS AAMERNKTKE QLEEEKKISL SFRIKPLAIE GFGEAKLREK AQELWELIVK LETEKYDLEE RQKRQDYDLK ELKERQKQQL RHKALKKGLD PEALTGKYPP KIQVASKYER RVDTRSYDDK KKLFEGGWDE ISKDSNEKIW NEKKEQYTGR QKSKLPKWFG ERPGKKAGEP ETPEGEEDAK ADEDIVEDDE EVEEEVVEEE DEEAEEDEEE EEEEEEEEEE EEEEEEEEEE EEEEEEE
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能參考文獻:
    1. The present study throws light on a new role played by miR-9a during muscle development and function, showing that miR-9a is involved in the translational regulation of TnT levels during sarcomeric assembly. PMID: 28866639
    2. Hypergravity centrifugation caused fast (i.e. within 24 h) changes to fly leg muscle TnT alternative splicing that correlated with body weight variation across eight D. melanogaster lines. Hypergravity treatment also appeared to enhance leg muscle function, as centrifuged flies showed an increased negative geotaxis response and jump ability. PMID: 28978639
    3. Report drosophila TNT mutation causing cardiac dysfunction and remodeling comparable to that observed during human restrictive cardiomyopathy. PMID: 24221941
    4. there are two modular enhancer elements in troponin T which establish the correct levels of Troponin T expression, adapting these levels to their specific needs PMID: 14718560
    5. In flies overexpressing a TnT transgene, the TnT increase is seen only in early stages of adult muscle formation, followed by a decrease in this & other thin-filament proteins, maybe by triggering down-regulation of co-regulated thin-filament components. PMID: 15469415

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  • 蛋白家族:
    Troponin T family
  • 組織特異性:
    Isoform 3 is expressed in the hypoderm. Isoform 8 is expressed in the dorsal vessel. Isoform 6 is expressed in adult TDT muscle and isoform 9 in adult IFM, flight and jump muscles.
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG7107

    STRING: 7227.FBpp0073682

    UniGene: Dm.20472



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